MIT-SCIENCE-Lectures-fa01quizrdmans.pdfVIP

7.02 Fall 2001 RDM Exam Name ___________KEY________________ 1 Question 1 (20 points) Please mark whether each of the following statements is true or false. If a statement is false, correct it by crossing out and/or substituting words or phrases. gray (For example: __ False__ The winter sky over Boston is usually blue). Grading was as follows: -1 point if true/false incorrect -1 point for failing to correct a false statement -1 point for correcting a true statement __false___ a) In an agarose gel, plasmid DNA that is supercoiled migrates more slowly than DNA that is nicked. Acceptable corrections (changes are underlined): 1. In an agarose gel, plasmid DNA that is supercoiled migrates more quickly than DNA that is nicked. 2. In an agarose gel, plasmid DNA that is nicked migrates more slowly than DNA that is supercoiled. __false___ b) Isopropanol was used to remove excess salt from precipitated miniprep DNA. 1. Isopropanol was used to precipitate miniprep DNA. 2. 80% Ethanol was used to remove excess salt from precipitated … DNA. Note: -0.5 points for Ethanol instead of 80% Ethanol; in fact, it is the 20% water in 80% ethanol that actually removes the excess salt. ___true___ c) Ligation of two fragments of DNA by T4 DNA ligase requires ATP. ___false__ d) AG1111 cells contain T7 RNA polymerase and will therefore express GFP. 1. AG1111 cells do not contain T7 RNA polymerase and therefore will not express GFP. 2. BL21 cells contain T7 RNA polymerase and will therefore express GFP ___false__ e) Ethidium bromide binds to DNA is a stoichiometric manner, i.e. the shorter the DNA fragment, the more molecules of EtBr it binds. 1. Ethidium bromide binds to DNA is a stoichiometric manner, i.e. the shorter the DNA fragment, the fewer molecules of EtBr it binds. 7.02 Fall 2001 RDM Exam Name ___________KEY________________ 2 2. Ethidium bromide binds to DNA is a stoichiometric manner, i.e. the longer the DNA fragment, the more molecules of EtBr it binds. ___