Labvariables.docVIP

Lab 1: Perl语言环境部署及变量练习 Objects: LINUX及WINDOWS Perl编程环境部署； 掌握在Linux环境下编写Perl程序； 掌握标量变量（scalars）的用法； 掌握数列变量（arrays）的用法； 掌握散列变量（hash）的用法。 PERL安装 LINUX：一般PERL编译环境在LINUX操作系统安装后即已安装好； WINDOWS：下载ActivePerl并按提示安装。 登录Linux服务器 使用Xming登录Linux服务器，点击保存的config文件，输入用户密码点完成。 使用gedit编辑Perl程序 在命令行中输入gedit 后回车(在命令后加上是让程序在后台运行，不然的话在编辑程序时命令行不能运行其他命令) 在gedit的编辑窗口编辑perl程序代码，注意选择View-Highlight Mode-Scripts-Perl选项，方便阅读程序代码。 程序运行 在命令行中输入perl 程序文件名 要求 完成A，B，C中任意一组题目，相关数据在public目录下。 Part A Assuming that there is an input file containing the PKC Beta-1 protein sequence and the file name is pkc_beta_1.seq. ?1. Using UNIX commands cat or more, display the PKC Beta-1 protein sequence contained in pkc_beta_1.seq. ?Write a Perl script and do the following: 2. Read in the PKC Beta-1 protein sequence from an external file and print the sequence on the screen. ?3. Count how many amino acids that the protein sequence contains. Print the number on the screen.(提示用length()) ?4. Given an average molecular weight of 110 daltons per amino acid, calculate the estimated molecular weight of the protein sequence in kilodaltons. Print the result on the screen.? Part B Assuming that there is an input file, dna_1.seq that contains a DNA sequence. Write a new Perl script and do the following: 5. Read in a DNA sequence from dna_1.seq and print the sequence on the screen.? 6. Transcribe this DNA sequence into an RNA sequence. Print both DNA and RNA sequences on the screen.?(提示用tr///) 7. This time, take the DNA sequence and calculate its reverse complement. Print the DNA sequence and its reverse complement on the screen.?(提示用tr///和reverse()) Part C Assuming that there are two input files, homo_sapiens_1.seq and homo_sapiens_2.seq both of which contain a DNA sequence to be translated into proteins. ?Write a new Perl script and do the following: 8. Read in a DNA sequence from homo_sapiens_1.seq and print the sequence on the screen. ?9. Count how many nucleotides the sequence contains and print the number on the screen. ?