生物化学——第一章蛋白质化学1幻灯片.ppt

* disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( * disease, food poisoning) disease, food poisoning) foodborne foodborne ( 五、蛋白质的呈色反应 ⒈茚三酮反应 蛋白质经水解后产生的氨基酸也可发生茚三酮反应。 ⒉双缩脲反应 蛋白质和多肽分子中肽键在稀碱溶液中与硫酸铜共热，呈现紫色或红色，此反应称为双缩脲反应，双缩脲反应可用来检测蛋白质水解程度。 （一）分离纯化蛋白质的意义 研究蛋白质的结构与功能：要求纯度高,不变性； 提取活性的酶或蛋白质：必须保持天然活性状态； 作为药物或食品添加剂：纯度要求一般。 （二）蛋白质分离纯化的一般步骤 材料的选择→原料的预处理→蛋白质的抽提→从抽提液中沉淀蛋白质→纯化→蛋白质的结晶 第七节 蛋白质