Acylic Sugar Derivatives for GCMS Analysis of.pdf

Subscriber access provided by ZHEJIANG UNIV OF TECH Analytical Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Article Acylic Sugar Derivatives for GC/MS Analysis of 13 C-Enrichment during Carbohydrate Metabolism Neil P. J. Price Anal. Chem., 2004, 76 (22), 6566-6574 ? DOI: 10.1021/ac049198m Downloaded from on November 26, 2008 More About This Article Additional resources and features associated with this article are available within the HTML version: ? Supporting Information ? Links to the 1 articles that cite this article, as of the time of this article download ? Access to high resolution figures ? Links to articles and content related to this article ? Copyright permission to reproduce figures and/or text from this article Acylic Sugar Derivatives for GC/MS Analysis of 13C-Enrichment during Carbohydrate Metabolism Neil P. J. Price* Departments of Pharmacology and Physiology, and Anesthesiology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York 14642-8711 Metabolic profiling with stable-isotope tracers in combina- tion with gas chromatography/mass spectrometry (GC/ MS) is a well-established technique for measuring sub- strate redistribution within metabolic pathways. This analysis relies on the ability to localize and quantify the fractional incorporation of 13C isotope into each carbon atom of precursor-derived metabolites. In this paper, several carbohydrate derivatization procedures (peracety- lation, deuterioalditol acetates, and aldononitrile acetates) are evaluated for the positional isotopic information obtained by gas chromatography/electron impact mass spectrometry (GC/EI-MS). These derivatives have been compared for the quantitative evaluation of 13C distribu- tion into isotopomers of 13C-labeled aldoses and ketoses, and the fragmentation pathways for 15 hexoses, pentoses, and amino sugars of biological origin have been assessed. In addition, a new