ibidi细胞定位格子培养皿.pdfVIP

ibidi细胞定位格子培养皿 μ-Dish 35mm Grid-500 35mm 具 500um 网格刻度 ibidi 标准底部的培养皿 产品特点： 1.特别适合细胞或细胞簇的定位和重找回； 2.独特的 ibidi 标准底部设计，180um 的盖玻片尺寸底部厚度，适合高分辨率显微观察； 3.独特的 ibiTreat 底部包被，适合大多数细胞的贴壁培养； 4.特殊盖部设计减小液体挥发。 应用： 1.细胞或细胞簇的再定位 2.细胞计数，例如：细胞转染效率计算 3.细胞运动观察 4.细胞显微操作中单细胞处理 技术特征： 1.直径为 35mm 标准样式的培养皿； 2.具 500μm 网格刻度； 3.字母或数字标记(A-U; 1-20)； 4.替代选择的玻璃底部产品：μ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500； 5.独特的 ibiTreat 组织培养处理表面适合大多数细胞的贴壁生长； 6.生物相容性材料制作，不含胶水，无细胞毒性影响； 7.特殊的盖部设计，减小液体挥发。 产品规格： Grid repeat distance 500 μm ? μ-Dish 35 mm Volume high / low 2 / 0.8 ml Growth area 3.5 cm 2 ? observation area 21 mm Height with lid high / low 14 / 9 mm Bottom: ibidi Standard Bottom Layout Grid-500 Lid with Lock Position The special lock feature in all ibidi μ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish. TIP: Use the locking feature only if minimal evaporation is required, i.e., outside incubators, non humidified microscopy stages, etc. ibidi μ-Dish Family: Versions and Variations μ-Dish 35 mm, high μ-Dish 35 mm, low μ-Dish 50 mm, low High walls: 标准应用 Low walls: 细胞显微操作 Ibi S ibidi standard Bottom vs. ibidi Glass Bottom ibidi Standard Bottom ibidi Glass Bottom Optical properties Refractive index (nD 589 nm) 1.52 1.52 Abbe number 56 55 Thickness #1.5 (180 μm) #1.5 (170 μm) Material Microscopy plastic D 263M Schott borosilicate glass Autofluorescence Low Low Transmission Very high (even ultraviolet) High (ultraviolet restrictions) Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*) Other aspects Surface modifications ibiTreat – tissue culture treated Uncoated – hydrophobic Only pure glass Protein coatings Possible Possible Gas pe