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2017-04-14 发布于天津
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Functional Genomics - Woods Hole Oceanographic ….ppt

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Functional Genomicsin Non-Model Organisms What is Functional Genomics? Functional genomics refers to the development and application of global (genome-wide or system-wide) experimental approaches to assess gene function by making use of the information and reagents provided by structural genomics. It is characterized by high-throughput or large-scale experimental methodologies combined with statistical or computational analysis of the results (Hieter and Boguski 1997) Functional genomics as a means of assessing phenotype differs from more classical approaches primarily with respect to the scale and automation of biological investigations. A classical investigation of gene expression might examine how the expression of a single gene varies with the development of an organism in vivo. Modern functional genomics approaches, however, would examine how 1,000 to 10,000 genes are expressed as a function of development. (UCDavis Genome Center) Functional GenomicsHunt Livesey (eds.) Subtracted cDNA Libraries Differential Display Representational Difference Analysis Suppression Subtractive Hybridization cDNA Microarrays Serial Analysis of Gene Expression 2-D Gel Electrophoresis My View of Functional Genomics Differential Gene expression SAGE/MPSS RDA/SSH *Open systems* Identifying the Function of Genes Functional Complementation RNA interference/RNA silencing Disclaimer Relevant primarily to eukaryotes Most common systems (literature/class) Personal experience with them I like them Why We Need Functional Genomics My Two Cents (as expressed by Hieter Boguski 97) Functional genomics will not replace the time-honored use of genetics, biochemistry, cell biology and structural studies in gaining a detailed understanding of biological mechanisms. The extent to which any functional genomics approach actually defines the function of a particular protein (or set of proteins) will vary depending on the method and gene involved. mRNA abundance classes(Okamuro Goldberg) Superabu