IonAmpliSeq外显子组文库制备.PDFVIP

Ion AmpliSeq qPCR                             ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐      DNAA   3    4    5    5    6    7    9    2                             ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐    IIon AmpliSeeq Exome kitt(Cat. no. 4487084, Toorrent Suite 4.002 Ion Amplisseq exome http://bit.lly/ampliseqexome. DNNA   TaqMMan® RNase P Detection Reagents Kit (Cat. no. DNAA Qubit dsDDNA HS asssay kit(Cat. nno. Q32851 oor Q32854)   1. (µL) 5X Ion AmmpliSeq™ HiFi Mix 14 50 100 ng ggDNA (non-FFFPE)* Y Nucleaase-free wateer (21-Y) Total 35 *DNAA 1100ng 2. 2.55µL 96 (Cat. no. N8010560 or12 3. 12 2.5µµL EExome Primmer Pool 1--12 5µLL.   3                             ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐    4. MicroAmmp® clear addhesive film (Cat. no. 96 5. MicroAmmp® Compreession Pad 96 96 PCCR liffe technologgies PPCR