乙酰胆碱对大鼠头端延髓腹外侧区前交感神经元放电的作用.pdf

　　Received 2000204224 　　Accepted 2000206208 　　3 Project supported by National Basic Program (No. G2000056905) of China and the Research Foundation for Medical Sciences of PLA (the 9th Five2Year Program No. 96M072) 　　3 3 Corresponding author. Tel : 021225070262 ; E2mail : yuanwj @smmu1edu1cn 　468 　 生理学报 , Dec. 2000 , 52 (6) : 468～472 Acta Physiologica Sinica 乙酰胆碱对大鼠头端延髓腹外侧区 前交感神经元放电的作用 3 王伟忠 , 汪雪梅1 , 戎伟芳 , 王继江 , 袁文俊 3 3 (第二军医大学基础医学部生理学教研室 , 上海 200433 ; 1 安徽省卫生职工培训中心 , 合肥 230000) 摘 　要 : 　实验采用细胞外记录和微电泳等电生理方法 , 研究乙酰胆碱 (ACh)对氨基甲酸乙酯麻醉的大鼠头端延 髓腹外侧区 (RVLM)前交感神经元放电频率的影响。在 RVLM共记录到 35 个前交感神经元 , 微电泳 ACh 能增加其 放电 ( P 0105) , 并且具有剂量依赖性。其中 22 个神经元微电泳 M型胆碱受体阻断剂阿托品 (ATR)后能明显降低 前交感神经元的基础放电 ( P 0105)和完全阻断 ACh 引起的神经元兴奋作用 ; 分别向其余 7 和 6 个单位微电泳筒 箭毒 (ACh N1 和 N2 型受体阻断剂)或六烃季铵 (ACh N1 型受体阻断剂)后均不能影响神经元的基础放电和 ACh 引起 的神经元兴奋作用。结果提示 , RVLM前交感神经元存在以 M型受体介导的胆碱能纤维的紧张性兴奋投射。 关键词 : 头端延髓腹外侧区 ; 前交感神经元 ; 细胞外记录 ; 微电泳 ; 乙酰胆碱 学科分类号 : Q425 Effect of acetylcholine on the discharge of presympathetic neurons of the rostral ventrolateral medulla of rats 3 WANG Wei2Zhong , WANG Xue2Mei 1 , RONG Wei2Fang , WANGJi2Jiang , YUAN Wen2Jun 3 3 ( Department of Physiology , Second Military Medical University , Shanghai 200433 ; 1 Training Center of Health Personnel , Hefei 230000) Abstract : 　 Effect of acetylcholine (ACh) on presympathetic neurons of the rostral ventrolateral medulla (RVLM) was studied by extracellular recording technique and microiontophoresis in urethane2anaesthetized rats. Spontaneous discharges of 35 presympathetic neurons of RVLM were recorded. All the neurons showed increased discharge by microiontophoresis of ACh dose2 dependently with the increase of iontophoretic current . In 22 units microiontophoresis of atropine decreased the baseline discharge rate and completely blocked the ex2 citatory effect of ACh on these neurons , while in the remaining 7 and 6 neurons microiontophoresis of d2tubocu2 rarine (d2TC) and hexamethonium (C6) respectively had no effect and did not block the action of ACh. Th