光照和激素对耐热型大白菜游离小孢子培养的影响.pdf

光照和激素对耐热型大白菜游离小孢子培养的 影响 X 盛 鹏1, 张秀荣2, 岳艳玲1 XX , 李晶晶 1 , 邓 静1 1.云南农业大学园林园艺学院, 昆明 650201; 2.松原市前郭县白依拉嘎乡农业技术推广站, 前郭 131113 摘 要: 将分离纯化的小孢子悬浮培养在添加 0, 011, 012, 013, 015, 110 mg/ L 的 NAA 和 0, 0105, 011, 012, 013, 015, 110 mg/ L 6-BA的 NLN培养基中 ,在 A( 3 000 lx 光培养)、B( 10 d暗培养再 3 000 lx光培养)、C( 15 d 暗培养 再 3 000 lx光培养)、D( ck,暗培养)条件下进行培养,研究光照和激素对耐热型大白菜/ 夏将军0游离小孢子成 胚及胚状体发育的影响。结果表明: 011 mg/ L 6-BA能显著促进胚状体的发生, 使胚产量达到最高, 为 206 个。 同时附加 012 mg/ L NAA 不但保证有较高的胚产量 ( 205 个) , 还可促进胚状体发育, 子叶胚及萌发胚可达 3317% ,畸形胚比例下降到1411% ;暗培养能促进成胚, D处理成胚量高,达 149 个,光培养则明显抑制成胚, 但 可促进胚状体发育,提高成苗率, 其中以 B 处理成胚成苗效果较好,成胚 108 个, 成苗率 145%。 关键词: 耐热型大白菜; 游离小孢子; 光照; 激素; 成胚; 成苗 中图分类号: S63411 文献标识码: A 文章编号: 1000-5684( 2011) 06-0649-05 DOI: CNKI: 22- 1100/ S. 1624. 003 网络出版地址: http: / / www. cnki. net/ kcms/ detail/ 22. 1100. S. 1624. 003. html 引文格式: 盛鹏, 张秀荣,岳艳玲, 等1 光照和激素对耐热型大白菜游离小孢子培养的影响[ J] . 吉林农业大学 学报, 2011, 33( 6) : 649-653. Effect of Different Light and Hormone Treatments on Isolated M-i crospore Cultures of Heat-Tolerance Chinese Cabbage SHENG Peng 1 , ZHANG Xiu-rong 2 , YUE Yan-ling 1 , LI Jing-jing 1 , DENG Jing 1 1. College of Landscape and Horticulture, Yunnan Agricultural University , Kunming 650201, China ; 2. Qianguo Baiyilaga Town Agro-Tech Extension Station of Songyuan City , Qianguo 131113, China Abstract: 0, 011, 012, 013, 015 and 110 mg/ L NAA and 0, 0105, 011, 012, 013 and 015, 110 mg/ L 6-BA were added to NLN media, and A-3 000 lx light, B-10 d dark then 3 000 lx light, C- 15 d dark then 3 000 lx light and D-dark were applied to culturing isolated microspore of hea-t tolerance Chinese cabbage-Xiajiangjiu in order to study the effect of light and hormone on the growth and induction of microspore-derived embryos. The results showed: 011 mg/ L 6-BA could improve induction of m-i crospor-e derived embryos. The highest yield, 206 embryos, was obtained. At the same t ime adding 012 mg/ L NAA could not only induce higher embryos, 205 embryos, but also promote growth of em- bryos . Cot