Flavo-ReductaseModelinWater.PDFVIP

Electronic Supplementary Material (ESI) for Dalton Transactions. This journal is ? The Royal Society of Chemistry 2016 Electronic Supplementary Information for Reduction of a Tris(picolyl)amine Copper(II) Complex by a Polymeric Flavo-Reductase Model in Water Synthesis. Flavin mononucleotide (FMN), riboflavin, and NADH, were purchased from commercial suppliers and used without further purification. The polyethyleneimine polymer modified with guanidinium and octyl groups was synthesised and purified as previously described from a 25 kDa multibranched polyethyleneimine polymer.[1,2] The tris(picolyl)amine copper(II) complex was synthesised and analysed by X-ray diffraction analysis as previously described in the literature.[3,4] Samples preparation. All deoxygenated samples were prepared under argon in a glove box before being transferred to cuvettes equipped with septa, or in EPR tubes sealed with parafilm. Electronic absorption spectroscopy. All experiments were carried out with a Varian Cary 300-bio or a Cary 100 spectrophotometer in cuvettes equipped with septa for experiments under inert atmosphere. EPR experiments. For X-band EPR measurements, a Bruker Elexsys E 500 EPR spectrometer equipped with an Oxford ESR9 liquid helium flow cryostat was used with a gold-chrome thermocouple positionned directly below the sample. The samples to be analysed (~150 μL) were prepared under argon in a glove box and transferred to EPR tubes with septa sealed with parafilm. The tubes were then rapidly immersed in an ethanol bath at ~ -100°C (ethanol cooled with liquid nitrogen) and were finally transferred into liquid nitrogen for cooling to 77 K before being introduced into the EPR spectrometer. This supporting information also includes: