环磷酸腺苷高产菌落研究生物工程定稿_毕业论文.doc

——学院 2014届本科毕业论文 论文题目：环磷酸腺苷高产菌落研究 学生姓名：—— 所在院（系）：生物工程系 所学专业：生物工程专业 导师姓名：—— 完成时间：2014年3月15日 环磷酸腺苷高产菌落研究 —— （——学院生物工程系104班） 摘 要 微生物发酵法合成环磷酸腺苷（cAMP）高产菌种是关键，本文采用高效液相色谱法测定不同菌落发酵液中环磷酸腺苷的含量筛选出cAMP高产菌落。通过活化不同节杆菌，挑选单菌落，分别编号1至6后移种进行发酵，发酵结束后用高效液相色谱法测定发酵液中cAMP含量,结果6号菌落发酵液中cAMP含量最高为2.8957mg/ml，其次为4号菌落，环磷酸腺苷含量为2.5057 mg/ml。本实验初步筛选出环磷酸腺苷高产菌落为6号菌落；同时也发现在实验中无菌操作是否规范、能否避免染菌是我们实验成败的关键。 关键字：环磷酸腺苷，节杆菌，发酵，高效液相 THE EFFECT OF：Using high performance liquid Cyclic adenosine phosphate high-yield strains screened —— (—— School, Department of Biological Engineering，Class 104) ABSTRACT Microorganism fermentation was synthesized adenosine monophosphate (cAMP) high yield strain is the key, this paper adopts high performance liquid chromatography (HPLC) method for determining the content of central adenosine phosphate of different colonies fermented liquid screened cAMP high-yield colony. Through activation of different section, select single colony, number 1 to 6 back kind of ferment, respectively after fermentation cAMP content in the fermented liquid by high performance liquid chromatography (HPLC) method, the results 6 colonies cAMP content in the fermented liquid up to 2.8957 mg/ml, followed by colony, 4 cyclic adenosine phosphate content is 2.5057 mg/ml. This experiment preliminary screening cyclic adenosine phosphate high-yield colony for 6 colonies; Also found in the experiment of sterile operation whether standard, whether to avoid dye bacterium is key of success or failure in our experiment. Key words: Cyclic adenosine monophosphate, Arthrobacterium ,Biological Fermentation，HPLC PAGE \* MERGEFORMAT16 目 录  TOC \o 1-3 \h \z \u  HYPERLINK \l _Toc383352226 1引言：  PAGEREF _Toc383352226 \h 1  HYPERLINK \l _Toc383352227 1.1环腺苷酸的物理化学性质和生物学功能  PAGEREF _Toc383352227 \h 1  HYPERLINK \l _Toc383352228 1.2环磷酸腺苷生产机制  PAGEREF _Toc383352228 \h 2  HYPERLINK \l _Toc383352229 2.材料与方法  PAGEREF _Toc383352229 \h 3  HYPERLINK \l _Toc383352230 2.1实验材料  PAGEREF _Toc383352230 \h