响应面法设计降低鸡蛋胆固醇中草药复方添加剂的研究.docVIP

响应面法设计降低鸡蛋胆固醇的中草药复方添加剂的研究收稿日期： 基金项目：国家自然科学基金资助项目；云南省科技创新强省计划资助项目（2014AB015） 作者简介：门文卉，1990.03，女，安徽人，硕士生，中草药及微生物在绿色循环农业中应用 通讯作者：邓先余，男，副教授，博士E-mail: dengxy1008@126.com 门文卉，陈晗，林连兵，邓先余* （昆明理工大学，生命科学与技术学院，云南省昆明市 邮编650500） 摘要：对泽泻、决明子、绞股蓝超微粉碎物用量进行优化组合，探索降低鸡蛋胆固醇的中草药复方添加剂配比，依据响应面法原理使用Box-Behnken设计实验数学模型并评估最优配比下中草药复方添加剂的效果。结果表明，中草药复方最佳配比为：泽泻超微粉碎物用量695.49mg，决明子超微粉碎物用量893.14mg，绞股蓝超微粉碎物用量334.13mg，该条件下检测鸡蛋胆固醇含量为2.48mmol/L，与空白对照相比，试验组的料蛋比降低了33.64%，产蛋率提高了11.73%，有显著性差异(p0.05)；显著降低了蛋黄中的胆固醇含量69.90%和甘油三酯含量41.78%(p0.05)，显著提高蛋鸡免疫能力(p0.05)。响应面法能够用于中草药复方添加剂处方配比优化，此配方能很好的降低鸡蛋胆固醇，为实际生产提供理论依据。 关键词：响应面；鸡蛋；胆固醇；中草药复方 中图分类号：S831.5 The study of compound Chinese herb additives optimization for reduction of egg cholesterol content by response surface method MENG Wen-hui, CHEN Han, LIN Lian-bin, DENG Xian-yu* （College of Life Science and Technology, Kunming University of Science and Technology, Yunnan650500, China） Abstract: The quantity of superfine comminution of Alisma orientale, semen cassia and Gynostemma pentaphyllum were optimized according to the mathematic model built by Box-Behnken Design on the rationale of response surface methodology and evaluate the effect of Chinese herb additives. The result was that: the best Chinese Herb Additives composition contained 695.49mg of superfine comminution of Alisma orientale, 893.14mg of superfine comminution of semen cassia, 334.13mg of superfine comminution of Gynostemma pentaphyllum. Under these conditions, the egg cholesterol concentrations was 2.48mmol/L. Compared with control group, the feed-egg rate was obviously dropped by 33.64%, the egg-laying rate increased obviously by 5.35%(p0.05), significantly reduce egg yolk cholesterol and triglyceride levels by 69.90%, 41.78%(p0.05), significantly increase the immune index(p0.05). Response surface methodology could be adopted for the optimize the ratio of Chinese herb additive formulation, which can lower cholesterol eggs well, provide a theoretical basis