积雪草苷的神经保护作用机制.doc

SICENCE新闻发布 作者：祁凤燕 稿号：NRR_143_14 积雪草苷的神经保护作用机制 体外研究发现积雪草苷具有神经保护作用，可以减轻β-淀粉样蛋白诱导的神经元损伤。然而，关于积雪草苷对谷氨酸诱导的兴奋性毒性的神经保护作用尚未见报道。来自中国第四军医大学齐凤燕所在团队研究结果证实，积雪草苷剂量依赖性抑制N-甲基-D-天冬氨酸诱导的神经元活力的降低，逆转N-甲基-D-天冬氨酸诱导的凋亡蛋白Bcl-2和Bax表达变化，显著逆转N-甲基-D-天冬氨酸受体NR2B亚基上调，但并不影响N-甲基-D-天冬氨酸受体NR2A亚基的表达，能明显抑制N-甲基-D-天冬氨酸诱导的细胞内Ca2+超载，以此发挥神经保护作用。实验结果为天然药物积雪草苷神经保护作用在临床的应用提供了新的实验数据。文章发表在《中国神经再生研究（英文版）》杂志2014年7月第13期。 Hoechst 33258和PI双重染色显示，积雪草苷预处理可抑制N-甲基-D-天冬氨酸诱导的小鼠大脑皮质神经元的凋亡 Article: Neuroprotective effects of Asiaticoside, by Feng-yan Qi1, Le Yang1, Zhen Tian1, Ming-gao Zhao1, Shui-bing Liu1, Jia-ze An2 (1 Department of Pharmacology, School of Pharmacy, Fourth Military Medical University of Chinese PLA, Xi’an, Shaanxi Province, China; 2 Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an, Shaanxi Province, China) Qi FY, Yang L, Tian Z, Zhao MG, Liu SB, An JZ. Neuroprotective effects of Asiaticoside. Neural Regen Res. 2014;9(13):1275-1282. 欲获更多资讯：HYPERLINK /Neural Regen Res Neuroprotective effects of Asiaticoside In the central nervous system, Asiaticoside has been shown to attenuate in vitro neuronal damage caused by exposure to β-amyloid. However, its potential neuroprotective properties in glutamate-induced excitotoxicity have not been fully studied. Researchers from Fourth Military Medical University of Chinese PLA, China reported that pretreatment with Asiaticoside decreased neuronal cell loss in a concentration-dependent manner and restored changes in expression of apoptotic-related proteins Bcl-2 and Bax. Asiaticoside pretreatment also attenuated the upregulation of NR2B expression, a subunit of N-methyl-D-aspartate receptors, but did not affect expression of NR2A subunits. Additionally, in cultured neurons, Asiaticoside significantly inhibited Ca2+ influx induced by N-methyl-D-aspartate. Their results provide a new insight into the neuroprotective effects of Asiaticoside. The relevan