急性肺缺血再灌注引起肺组织表面活性物质相关蛋白A变化实验研究.docVIP

KKME---专业医学搜索引擎/ 急性肺缺血再灌注引起肺组织表面活性物质相关蛋白A变化实验研究 　　作者：王海英，倪松石1 许文景2 作者单位：(1南通大学附属医院呼吸内科，南通226001;2苏北第一人民医院) 　　【摘要】 目的：探讨肺急性肺缺血再灌注(ischemia reperfution，I/R)时表面活性物质相关蛋白A(surfactant- associated protein A，SP-A)含量的变化与肺损伤的关系。方法： 40只健康的SD大鼠，雌雄不拘，随机分成5组：假手术组(S)、单纯缺组(I)、再灌注1 h组(R1组)、再灌注2 h组(R2组)、再灌注6 h组(R3组)。根据Eppinger模型建立原位阻断左肺门大鼠温肺I/R模型。术毕测定各组血清丙二醛(MDA)及肺湿/干重比例(W/D)、病理观察、用Western-Blot及免疫组化法检测各组肺组织中SP-A。结果：(1)MDA、肺W/D在I组即较S组开始升高，予再灌注后，各组MDA、肺W/D随再灌注时间的延长明显升高(P0.05);(2)SP-A在S组表达最多，在I组表达减少，再灌注后随时间的延长表达更少(P0.05)。结论： 大鼠肺急性I/R后，肺表面活性物质相关蛋白A减少是造成肺一系列损害的重要因素。 　　【关键词】 缺血再灌注;丙二醛;肺湿/干重;肺表面活性物质相关蛋白A 　　The Experimental Research of the change of surfactant- associated protein A Resulting 　　from the Acute Lung Ischemia-reperfution 　　WANG Haiying1, NI Songshi1 , XU Wenjing2 (1Department of respiratory Medicine, the Affiliated Hospital of Nantong University, Nantong 226001) 　　[Abstract] Objective：We detect the quantity of MDA in serum, the W/D, the quantity of SP-A in the lung tissue, In order to know the change of SP-A and the relation between the SP-As alternation and the injury. Methods：Without considering of their sex, 40Health SD rats were chosened and divided into five groups, 8 rats in every group. They were as follows: the rats who received a left anterolateral thoracotomy without clamping the left hilum as Sham group(S)，the rats whose left hilums were clamped for 1 hour without reperfusion as ischemia-only group(I)，the rats whose hilums were clamped for 1 hour and then unclamped for 1, 2, 6 hours as ischemia/reperfusion group (R1, R2, R3). The rat model of warm ischemia and reperfusion in situ in left lung is set up on the basis of Eppingers Model. After operation, MDA and lung W/D was detected for every group. The expression of SP-A in the lung tissue of every group was detected by Western-blot and immunohistochemistry. Results：(1)MDA and W/D was increased in I group. In R1, R2, R3 group, MDA and W/D was more as the time passed by. The difference among ever