白藜芦醇对大鼠重症急性胰腺炎胰腺组织COX（学术论文）.docVIP

KKME---专业医学搜索引擎/ 白藜芦醇对大鼠重症急性胰腺炎胰腺组织 COX2和iNOS表达的影响 　　作者：李琦，沙焕臣，马清涌，张 磊，张庆元 作者单位：西安交通大学医学院第一附属医院肝胆外科，陕西西安 710061 　　【摘要】 目的 探讨白藜芦醇(resveratrol, RES)改善重症急性胰腺炎(severe acute pancreatitis, SAP)大鼠胰腺微循环的作用机制。方法 SD大鼠随机分为3组：假手术(sham operation, SO)组、重症急性胰腺炎(SAP)模型组、白藜芦醇治疗(RES)组。以40g/L牛黄胆酸钠0.1mL/100g胆胰管逆行注射建立SAP模型。RES组术后立即经阴茎背静脉注射RES(20mg/kg)。分别于术后3、6、12h取材，进行胰腺组织学评分;检测胰腺组织环氧化酶2(cyclcoxygenase2, COX2)、诱生型一氧化氮合酶(inducible nitric oxide synthase, iNOS)的表达和一氧化氮(nitrogen monoxide, NO)含量。结果 RES组胰腺损伤较SAP组明显减轻(P0.05)。SAP组COX2、iNOS的表达和NO含量在各时间点均显著高于假手术组(P0.05);RES组COX2的表达在6、12h较SAP组明显下降，iNOS和NO在各时间点均较SAP组明显下降，差异具有统计学意义(P0.05)。结论 白藜芦醇可能通过下调COX2和iNOS的表达改善胰腺微循环。 　　【关键词】 重症急性胰腺炎;白藜芦醇;微循环;COX2;iNOS 　　in rats with severe acute pancreatitisLI Qi, SHA Huanchen, MA Qingyong, ZHANG Lei, ZHANG Qingyuan 　　(Department of Hepatobiliary Surgery, the First Affiliated Hospital, 　　Medical School of Xian Jiaotong University, Xian 710061, China)ABSTRACT: Objective To explore the possible mechanism of resveratrol (RES) in improving pancreatic microcirculation in rats with severe acute pancreatitis. Methods SO rats were randomly divided into 3 groups: shamoperation (SO) group, severe acute panceatitis (SAP) group, and resveratroltreated (RES) group. SAP model was made by injecting 40g/L sodium taurocholate at a dosage of 0.1mL/100g into the biliopancreatic duct. RES (20mg/kg) was given intravenously after modeling. All rats were sacrificed at 3, 6, and 12 hours after the operation. The pathological changes of pancreatic tissues were observed and scored under microscope. The expressions of cyclcoxygenase2 (COX2) protein and inducible nitric oxide synthase (iNOS) protein in pancreatic tissues were detected by immunohistochemistry. The level of nitrogen monoxide (NO) in pancreatic tissues was measured by NO detection kit. Results Microscopically, there were apparent pancreatic mesochymal congestion, edema, acinar necrosis and leukocytic infiltration from 3h to 12h after SAP m