2%%%结肠癌干细胞miRNA上海交大生物化学与生理学通讯.pdfVIP

Biochemical and Biophysical Research Communications 404 (2011) 273–278 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: /locate/ybbrc MicroRNA expression pro?le of colon cancer stem-like cells in HT29 adenocarcinoma cell line ? Huanle Zhang a, Weihua Li b, Feifei Nan a, Fang Ren a, Hongxia Wang a, Yingchun Xu a, Fengchun Zhang a, a Department of Oncology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Dongfang Road 1630, Shanghai 200127, China b Department of Pharmacology and Reproduction Toxicology, Shanghai Institute of Parenthood Research, Xietu Road 2140, Shanghai 200032, China article info abstract Article history: Increasing evidence has suggested cancer stem cells (CSCs) are considered to be responsible for cancer Received 23 November 2010 formation, recurrence, and metastasis. Recently, many studies have also revealed that microRNAs (miR- Available online 2 December 2010 NAs) strongly implicate in regulating self renewal and tumorigenicity of CSCs in human cancers. How- ever, with respect to colon cancer, the role of miRNAs in stemness maintenance and tumorigenicity of Keywords: CSCs still remains to be unknown. In the present study, we isolated a population of colon CSCs expressing MicroRNA a CD133 surface phenotype from human HT29 colonic adenocarcinoma cell line by Flow Cytometry Cell Cancer stem cells