N、P、K 异质含量拟南芥试管苗的表型适应特性.pdfVIP

广 西 植 物 Guihaia Nov．2014，34(6)：869—873 http：／／journa1．gxzw．gxib．cn DOI：10．3969／j．issn．1000—3142．2014．06．024 魏鹏，刘彤．N、P、K异质含量拟南芥试管苗的表型适应特性EJ3．广西植物，2014，34(6)：869—873 Wei P，Liu T．Phenotypic adaptive properties of Arabidopsis thaliana test—tube plantlets inoculated in the medium with heterogeneous content of N，P and K[J]．Guihaia，2014，34(6)：869 873 N、P、K异质含量拟南芥试管苗的表型适应特性 魏 鹏 ，刘 彤 (1．宁夏职业技术学院 植物组培快繁中心，银川 750002；2．新疆兵团绿洲生态农业重点实验室 ，新疆 石河子 832003) 摘 要 ：以模式种拟南芥为试验材料 ，有别于 自然环境下生长 的拟南芥 ，借助于组织培养方法利用均匀设计 对培养基中 KNO。、NH NO 、KH。P() 设置处理，探讨试管环境下拟南芥生长发育特性及对营养离子需求吸 收特点 ，结果显示拟南芥本性地通过加强莲座叶与茎生叶的功能以增加生殖生长期的分枝数与苔高 ；生殖生 长期影响试管苗茎生叶数 、苔高的离子种类增多，离子的交互作用更加复杂。通过分析模式植物对 N、P、K异 质含量培养基的表型适应特点可推论同一植物在不同时期使用单一培养基是不合理的。 关键词 ：拟南芥 ；试管环境；表型适应；营养元素；苗子质量 中图分类号：Q945．97 文献标识码 ：A 文章编号：1000—3142(2014)06—0869—05 Phenotypic adaptive properties of Arabidopsis thaliana test-tube plantlets inoculated in the medium with heterogeneous content of N，P and K W EI Peng ，LIU T0ng (1．Cent r 0，Plant Tissue Culture arm Rapid Propagation，Ningccia Polytechnic College，Yinchuan 750002，China； 2．Key Laboratory of Oasis Ecological Agriculture 0_，xi iang Regiments，Shihezi 832003，China) Abstract：Characteristics of growth and development along with the specialty and the demand of absorbing nutritional ions of Arabid0psis thaliana in vitro were discussed by setting mode plant A．thaliana as the experimental material， unlike natural growth of Arabidopsis and by setting different levels of KNO3，NH4 NOn and KHz P()4 of treatment medium with in virtue of tissue culture technology and uniform design．The results showed that A．thaliana plantlets would reinforce the physiological function of rosette leaf and cauline leaf to enlarge the branches and the height of trunk：In reDroductive phase，ion species which affect the number of cauline leaf and the height of trunk increased，the interaction of minera1 ions was more complicated．Through analyzing photo-adaptive properties of Arabidopsis thali— aria test tube Dlantlets inoculated on t