两种细胞系用于建立体外脂肪肝模型的比较的论文.docVIP

　　两种细胞系用于建立体外脂肪肝模型的比较的论文 作者：潘雪丰，温彩霞，许建华 【摘要】 目的 对比油酸诱导肝癌细胞株hepg2、正常肝细胞株l02建立的肝细胞脂肪变性模型,通过检验复方蛋氨酸胆碱的防治作用，寻找简便、稳定的体外药物筛选模型。方法 设正常组、hepg2模型组、hepg2不同浓度给药组、l02模型组、l02不同浓度给药组、用油酸诱导肝细胞脂肪变,复方蛋氨酸胆碱干预，以油红o染色镜下观察细胞内脂滴形成状况,并检测细胞上清中的alt、ast、γgt水平。结果 油酸刺激hepg2、l02 肝细胞24 h,细胞内典型脂肪滴形成。复方蛋氨酸胆碱2次给药，药物难以使hepg2细胞内脂滴减少，l02细胞在加油酸48 h后油红o 染色证明细胞内脂滴可减少，脂滴表达的红色iod值，各给药组与模型组比较，差异有极显著性(plt;0.01)。细胞上清液中alt、ast、γgt，复方蛋氨酸胆碱各组与模型组比较差异有显著性，证实其对肝细胞损伤较小。结论 l02比hepg2更适合作为体外的筛药细胞模型，油酸刺激l02细胞形成脂肪变，药物提前24 h干预，24 h后再给药1次，这种造模和给药方案可作为一种可行的脂肪肝细胞筛药模型使用。. 【关键词】 肝癌细胞株hepg2;l02肝细胞株;油酸;脂肪肝;模型;体外;复方蛋氨酸胆碱 abstract:objective to pare todels of hepatocytic steatosis in vitro，a cell hepg2 and human liver cell l02 ination the role of pound methionine and choline bitartrate,to find a convenient and stable model in vitro for drug screening.methods normal group,hepg2 model group,groups of hepg2 cells treated ethionine and choline bitartrate at different concentrations,l02 model group,and model groups of l02 cells treated odels icroscope. alt,ast,and γgt in cell supernatant icroscope. ethionine and choline bitartrate,the amount of lipid droplet in hepg2 cells did not decrease,but decreased in l02 cells after treated odel group,the integral optical density (iod) value of positive of red fatty droplets in the group treated arkedly (plt;0.01). there ethionine and choline bitartrate and model groups,indicating that the damage to hepatocyte l02 all.conclusion l02 cell line ore suitable in the developing of in vitro model of hepatocytic steatosis,the model ethionine and choline bitartrate for 24 hours,and treated ethod could be used to develop an in vitro model of hepatocytic steatosis model for drug screen. 　　key a cell hepg2; human liver cell l02; oleic acid; fatty liver; model; in vitro; pound methionine and choline bitartrate 　　近年来,非酒精性脂肪性肝病(nafld)的发病率不断上升,由于它是肝硬化、肝癌的重要病因[1,2],已成为肝病领域的新挑战。目前对脂肪肝的研究主要采用的是动物模型，存在个体差异较大、实验条件不好控制、周期较长、材料耗费较多等缺点。而细胞模型实验却可以很好地克服上述缺点，不仅能针对性地探究细胞水平的脂肪肝发病机制，并且能有效地缩短筛选 治疗 药物的进程，有