他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响的论文.docVIP

　　他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响的论文 作者：宫惠琳，李扩，张冠军，侯惠莲，董炜疆 【摘要】 目的 观察他汀类药物对神经细胞和神经胶质细胞atp结合盒式转运体1(atpbinding cassette transporter 1, abca1)基因表达的影响。方法 培养人类神经细胞和神经胶质细胞，分别加入不同浓度的辛伐他汀(simvastatin)和普伐他汀(pravastatin)，孵育不同时间后分别提取细胞总rna和胞质蛋白，rtpcr观察abca1基因的表达，ethods primary human astrocytes and neuron cell line h2 vastatin and pravastatin at different concentrations and under different conditions. using rna isolated from the cultured cells, ed quantitative pcr to measure the abca1 gene expression in astrocytes and neurons. the protein expression of abca1 easured by vastatin significantly reduced the