CCR9-CCL25介导小鼠经生殖道hCGβ避孕疫苗粘膜免疫后B细胞的论文.docVIP

　　CCR9/CCL25介导小鼠经生殖道hCGβ避孕疫苗粘膜免疫后B细胞的论文 【摘要】 目的：探讨hcgβ粘膜避孕疫苗体液免疫的分子机制。方法：分别用1010个重组乳酸杆菌lb.pilac、lb. pilachcgβ经小鼠生殖道粘膜途径免疫6～8周龄balb/c小鼠，3周后相同剂量加强免疫1次。间接elisa检测加强免疫后2周阴道灌洗液中抗hcgβ igg和iga抗体滴度；elispot检测分泌抗hcgβ igg和iga抗体分泌细胞数；rtpcr筛查小鼠子宫b细胞上18种趋化因子受体和相关趋化因子ccl25；fcm和elisa分析ccr9/ccl25的表达。结果：经小鼠生殖道粘膜接种1010 lb. pilachcgβ可在生殖道局部诱导产生抗hcgβ igg和iga抗体, 且以igg抗体为主；局部存在抗hcgβ igg和iga抗体分泌细胞，而以igg 抗体分泌细胞为主；粘膜免疫后，小鼠生殖道局部b细胞上ccr9表达增高，粘膜局部相应配体ccl25表达亦增高。结论：重组乳酸杆菌活疫苗lb. pilachcgβ经小鼠生殖道粘膜免疫可诱导有效的免疫应答；粘膜局部ccr9/ccl25可介导b细胞归巢至生殖道局部，这可能增强hcgβ粘膜避孕疫苗的避孕效果。 【关键词】 hcgβ 粘膜免疫 ccr9/ccl25 b细胞 归巢 　　ccr9/ccl25 engaged in b cell homing at murine genital tract after immunization via vagina ingqing，yao xiaoying,li dajin. 　　laboratory for reproductive immunology，hospital institute of obstetrics gynecology，fudan university shanghai medical college，shanghai 200011，china 　　［abstract］ objective:to investigate the mechanism for b cell homing in murine genital tract after inoculation of lactobacillus expressing hcgβ via vagina.methods:balb/c mice munization ined by indirect enzymelinked immunosorbent assay(elisa). the igg/iga antibody producing cells(ascs) levels in all the immunized groups okine receptors in murine uterine b cells of both groups . the levels of ccl25 in uterus munization could be detected, and antihcgβ igg antibody ajor one. the levels of igg and iga ascs of lb.pilachcgβ immunization urine genital tract significantly increased after immunization ucosal immunization urine vagina can induce effective immune responses, and the specific antihcgβ antibody may be secreted by ascs in genital tract. ccr9/ccl25 is involved in the homing of b cells into murine genital tract, ight lead to optimal contraceptive efficiency of hcgβ mucosal vaccination. ［key ucosal immunization；ccr9/ccl25；b cell；homing 　　粘膜是机体与外界接触的重要屏障，也是外源抗原进入机体的主要门户。.粘膜免疫系统包括那些与身体内表面相关的粘膜相关淋巴组织（malt）,分布在胃肠道、上下呼吸道和泌尿生殖道,它面积巨大,仅分布于人的胃肠道的面积就有400平方米, 200倍于皮肤表面。健康成人malt中的免疫细胞占全身免疫细胞总数的80%，由此可见粘膜是人体的最大免疫器官。粘膜免疫系统担负着哨兵的责任，区分无害与有害