Sodium-borate(SB)vs.TBEforgelelectrophoresisByusing.pptVIP

Sodium-borate (SB) vs. TBE for gel electrophoresis By using SB for both gel and running buffer, we can: 1) Run gels faster at higher-throughputs 2) Get better quality gels -- Sharper bands -- Fully separated bands in 100-bp DNA ladder -- Better sample band separation size estimates -- Harder to overload with DNA 3) Save $ -- TBE = $0.54/L (from 10X Liquid Concentrate 4L, Amresco) -- SB = $0.11/L (from disodium borate decahydrate powder, 500 g, Fisher);Overview of Talk – 30 slides PCR product: 543 bp Alu (Owl monkey). -- Compare TBE SB at various voltages run