SodiumPhenylacetate(NaPa).PDF

ANTICANCER RESEARCH 27: 953-958 (2007) Sodium Phenylacetate (NaPa) Improves the TAM Effect on Glioblastoma Experimental Tumors by Inducing Cell Growth Arrest and Apoptosis MING X. WEI1, JIAN MIAO LIU2, FRANCK GADAL1, PING YI2, JIANFENG LIU2 and MICHEL CREPIN1,3 1Laboratoire d'Oncologie Moléculaire et Cellulaire, SMBH, Université Paris XIII. 74 rue Marcel Cachin, 93017 Bobigny; 3Inserm U553, Hopital Saint Louis, 1 Avenue Claude Vellefaux 75010 Paris, France; 2Institute of Biophysics and Biochemistry, College of Life Science, HuaZhong University of Science and Technology, 1037 LuoYu Road, Wuhan, 430074, P.R. China Abstract. Background: Multiform glioblastomas represent Malignant gliomas represent about 40% of all primary brain the most aggressive brain tumors. Here, the cooperative effects tumors in humans (1). They are considered incurable and of sodium phenylacetate (NaPa) and/or tamoxifen (TAM) on even multimodal approaches, including surgery, radiation CNS1 and 9L glioblastoma cell lines in vitro and in an therapy and chemotherapy, prolong the survival of patients experimental animal tumor model were investigated. Materials by only a few months (2). This resistance to therapy is due and Methods: The drug effects on cell cycle and apoptosis in part to glioma cell migration, phenotypic heterogeneity were investigated by flow cytometry. CNS1 cells were and a very poor immune response in the brain, as well as to implanted subcutaneously in nude mice to form tumors which difficulties in chemotherapeutic agents passing through the were then treated with NaPa, TAM or NaPa/TAM. Results: A blood-brain barrier. Therefore, new approaches to therapy significant inhibitory effect of NaPa on the two glioma cell of these tumors should be evaluated, such as combined –5 lines (LD50 of 10 mM