樟树内生细菌EBS05的鉴定及其抗菌活性物质性质的研究.PDF

微生物学通报 JUL 20, 2009, 36(7): 988~993 Microbiology ? 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 樟树内生细菌 EBS05 的鉴定及其抗菌 活性物质性质的研究 文才艺* 尹志刚 陈建光 李洪连 (河南农业大植物保护学院 河南 郑州 450002) 摘 要: 在形态学分类的基础上, 通过 16S~23S rRNA ISR(Intergenic spacer regions)序列分析, 对 樟树内生细菌EBS05 进行了分类鉴定, 结果表明EBS05 为枯草芽孢杆菌。测定了EBS05 代谢活性 物质的理化性质, 结果表明, 活性组分在λ213.5 nm处有最大光吸收峰; 在pH 5~8 范围内其抗菌活 性稳定, 当pH＜4.0 或pH＞9.0 时, 抗菌活性显著降低; 热稳定性良好, 60°C~80°C处理 2 h后, 抗菌 活性不变, 1×105 Pa灭菌 30 min后, 抗菌活性仍然保持在 65%以上; 具有较强的抗紫外线照射能力, 对蛋白酶K不敏感; 具有较好的醇溶性, 易溶于甲醇和乙醇, 不溶于乙酸乙酯、乙腈和石油醚等有 机溶剂。 关键词: 植物内生细菌, 枯草芽孢杆菌, 16S~23S rRNA 间隔区, 抗菌活性 Identificition of Endophytic Bacteria EBS05 from Cinamonum camphra and Property of Its Antimicrobial Compound WEN Cai-Yi* YIN Zhi-Gang CHEN Jian-Guang LI Hong-Lian (College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan 450002, China) Abstract: An endophytic bacterium strain EBS05 from Cinamonum camphra was identified as Bacillus sub- tilis by morphological taxonomy and sequence analysis of 16S~23S rRNA intergenic spacer regions. Proper- ties of antimicrobial compound produced by EBS05 were assayed. The active compound had the maximum absorbance peak at λ213.5 nm. The antimicrobial activity was stable in solution with pH value from 5 to 8, and decreased significantly in solution with pH value less than 4.0 or more than 9.0. The antimicrobial com- pound had t