川芎嗪注射液对高糖诱导后人腹膜间皮细胞Ⅰ型胶原、基质金属蛋白酶1和金属蛋白酶组织抑制剂1表达的影响的论文.docVIP

　　川芎嗪注射液对高糖诱导后人腹膜间皮细胞Ⅰ型胶原、基质金属蛋白酶1和金属蛋白酶组织抑制剂1表达的影响的论文 【摘要】 目的：研究川芎嗪注射液对体外培养的人腹膜间皮细胞（human peritoneal mesothelial cells, hpmcs）在高糖刺激下ⅰ型胶原、基质金属蛋白酶1（matrix metalloproteinase1, mmp1）和金属蛋白酶组织抑制剂1（tissue inhibitor of metalloproteinase1, timp1）表达的影响。方法：胰蛋白酶消化法从人腹膜组织中分离间皮细胞进行原代培养并传代，分为5组（每组设3个样本）：正常组（完全培养液）、高糖对照组（2.5％葡萄糖）和高糖（2.5％葡萄糖）加低、中、高剂量川芎嗪（10、20和40 mg/l）组。采用半定量逆转录聚合酶链反应（reverse transcriptionpolymerase chain reaction, rtpcr）法检测细胞内ⅰ型胶原、mmp1和timp1 mrna表达；酶联免疫吸附测定（enzymelinked immunosorbent assay, elisa）法检测细胞上清液中ⅰ型胶原、mmp1和timp1的蛋白质水平，用二喹啉甲酸（bicinchoninic acid, bca）蛋白检测法测定细胞中蛋白质含量，用以校正elisa结果。结果：川芎嗪能显著降低高糖所致的hpmcs ⅰ型胶原和timp1的表达（plt;0.05，plt;0.01），两者在蛋白质和基因水平均呈量效关系；且中、高剂量川芎嗪能显著增加mmp1的表达（plt;0.05）。.结论：川芎嗪能减少hpmcs高糖刺激下ⅰ型胶原的合成，并且通过调节mmp1/timp1的平衡，促进ⅰ型胶原降解。 【关键词】 川芎嗪注射液; 腹膜间皮细胞; 高糖; ⅰ型胶原; 基质金属蛋白酶1; 基质金属蛋白酶抑制剂1; 体外实验 zhu gs, he js. j chin integr med. 2009; 7(1): 6569. received june 18, 2008; accepted july 22, 2008; published online january 15, 2009. indexed/abstracted in and full text linkout at pubmed. journal title in pubmed: zhong xi yi jie he xue bao. free full text (html and pdf) is available at . fopen access effects of ligustrazine injection on high glucoseinduced type ⅰ collagen, matrix metalloproteinase1 and tissue inhibitor of metalloproteinase1 expressions in human peritoneal mesothelial cells in vitro guisong zhu, jinsong he department of nephrology, the affiliated drum toedical school, nanjing, jiangsu province 210008, china objective: to investigate the effects of ligustrazine injection on type ⅰ collagen, matrix metalloproteinase1 (mmp1) and tissue inhibitor of metalloproteinase1 (timp1) expressions in human peritoneal mesothelial cells (hpmcs) cultured in high glucose conditions. methods: hpmcs human omenta by trypsin digestion method and subcultured. then, the hpmcs al control group, high glucose group and high glucose plus loedium and highdose ligustrazine (10, 20 and 40 mg/l ligustrazine respectively) gro