125I radionuclide coupled with nerve growth factor and Optimization.docVIP

 PAGE \* MERGEFORMAT 12 125I radionuclide coupled with nerve growth factor and Optimization Of: Lupei Song, Yuan Zhicheng, Yang Yong, Gong Aihua, Zhang Zhijian, Zhan Li Ping, Wang Peng, Bo Li Qiaoyu, cloud cover [Abstract] Objective: To investigate the radionuclides 125I and nerve growth factor (nerve growth factor, NGF coupling optimal reaction conditions. Methods: The method for linking markers and nerve growth factor and 125I-coupling reaction, a fixed feeding amount in the 125I 37 MBq of the conditions, changing the feeding amount NGF were 20,50,100,150 g, the same reaction conditions to trichloroacetic acid precipitation method yields. the product stored at 4 , the 1,2,3,5, 7 days 2,3,4 weeks radioactive detection of chemical purity of the product, evaluation product of in vitro stability. RESULTS: NGF 20 g feeding amount increased to 50 g by the time, 125I NGF production rate increased rapidly to 76.6% 79.0%, when the NGF continued to increase when the feeding amount to 150 g, 125I NGF production rate increased by 1.2%. After observation for 4 weeks, the product of 125I NGF labeling method 4 in vitro storage conditions without obvious iodothyronine, radiation always greater than 80% purity. Conclusion: 125I nerve growth factor coupled with the ideal ratio of raw materials for the 37 MBq 125I react with the 50 g NGF, a product of the conditions of radioactive 125I NGF concentration of 97.31 MBq / ml, radioactivity activity was 584.6 MBq / mg. [Keywords:] of radioactive nuclides, nerve growth factor, coupling notation [Abstract] Objective: To explore the optimal reaction conditions of radionuclides 125I labeling nerve growth factor (NGF). Methods: NGF was radiolabeled with 125I by indirect iodination. Keep the constant dose of 125I to be 37 MBq, the dose of NGF varied as 20, 50, 100, 150 g, yield was figured out by TCA precipitation. Keep product in 4 , radiochemical purity at 1 d, 2 d, 3 d, 5 d, 7d, 2 w, 3 w, 4 w was detected by TCA precipitat