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186 Pseudomonas aeruginosa β lactamases and drug sensitivity analysis
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186 Pseudomonas aeruginosa β lactamases and drug sensitivity analysis
【Abstract】 Objective: To understand the resistance of Pseudomonas aeruginosa to antibiotics in an effort to provide a basis for clinical treatment. Methods: Clinical samples were isolated in different site of infection to the 186 Pseudomonas aeruginosa to β lactamase detection and drug sensitive test results analysis. Results: 186 P. aeruginosa tested 11 types of antibiotic resistance for more than 5 are 72, accounting for 38.7%, of which β -lactamase-positive 60, accounting for 32.4%, β within the acyl amine-negative were 12, accounting for 6.3%. Conclusion: β lactamase producing Pseudomonas aeruginosa resistance to more serious, is currently the treatment of Pseudomonas aeruginosa imipenem drug of choice, followed by amikacin and ceftazidime.
Keywords: Pseudomonas aeruginosa; β lactamase; drug resistance
1 Materials and methods
1.1 Materials
1.1.1 The specimen sources hospital in January 2003 ~ December 2006 in patients with sputum, pharyngeal secretions, wound secretions, urine, blood and other samples isolated from 186 Pseudomonas aeruginosa.
1.1.2 Quality control strains of Pseudomonas aeruginosa ATCC 27853.
1.1.3 Antibiotics paper ceftazidime, gentamicin, piperacillin, cefoperazone, amikacin, aztreonam, ciprofloxacin, imipenem, cefotaxime, chloramphenicol, cotrimoxazole 11 species, both French bio bioMérieux products.
1.1.4 Identification of bacteria French biotechnology company bioMérieux ATB automatic bacteriological analyzer.
1.1.5 M H sensitivity agar Reagent Co., Ltd Hangzhou Tianhe microbial products.
1.2 Methods
1.2.1 bacterial culture according to the national clinical laboratory operating procedures (2nd edition) carried out, will take to obtain the pure culture colonies in French biotechnology company bioMérieux ATB automatic analyzer for identi
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