2 cases of formalin-fixed paraffin-embedded tissue of the STR test.docVIP

 PAGE \* MERGEFORMAT 5 2 cases of formalin-fixed paraffin-embedded tissue of the STR test Of: Zhao Lei, Zhaoxing Chun, Zhang Qingxia, Ye Jian, Liu Yacheng [Keywords:] fixed in formalin; paraffin-embedded tissue; MiniSTR In forensic practice, will meet on formalin-fixed paraffin-embedded tissue for individual identification cases. In general, from formalin-fixed paraffin-embedded tissues extracted DNA to undergo a series of red tape, can easily lead to DNA loss and degradation of these samples easily, making the test more difficult. 1 Case 2 cases of formalin paraffin embedded tissue, liver tissue, respectively, and chorionic villi. Paraffin-embedded tissue blocks from the test up to two years. Requested DNA testing and inspection with the corresponding blood samples for comparison. 2 test methods and results 2.1 dewaxing and hydration of paraffin-embedded sections cut above 10.0m thick tissue sections 10, with blades remove excess tissue around the paraffin into the Eppendorf 1.5ml centrifuge tube and add 1ml of xylene at room temperature dewaxing, 2h after the discard the liquid suction tube, again adding 1ml xylene, at room temperature dewaxing 12h. were dehydrated with ethanol 2 times, 95% ethanol, 75% ethanol, 50% ethanol, 25% ethanol dehydration, the 1st, each step of the interval 2h, each time adding 1ml. Finally, 1ml sterile deionized water wash pieces of tissue, 1. 2.2 digestion flakes PercollTM (Amersham Biosciences Corporation) separated liquid 400l, 20mg/ml proteinase K 30l, 10% SDS 40l, digested at 37 for 24h. During an additional 20mg/ml proteinase K 60l. 2.3 DNA extraction and purification using organic extraction of DNA, all of the extracted DNA solution, placed MicroconTM100 purification column (Millipore Corporation), 2500rpm centrifuge 20min, and then purified by a column upside down in a clean 1.5ml Eppendorf tube, 3500rpm centrifuge 10min, liquid collection tube for amplification. 2.4 PCR amplification were sep