32P colloids in primary cultured rat prostate cells in radiation research.docVIP

 PAGE \* MERGEFORMAT 11 32P colloids in primary cultured rat prostate cells in radiation research Of: the right to Xia Cao Yan Gu Kong Xiangbo Ma Qingjie [Abstract] Objective To observe the different concentrations of colloidal 32P in primary cultured rat prostate cell apoptosis. Methods Primary cultures of rat prostate cells, is divided into high-dose radiation group, low dose radiation group and the control group, using flow cytometry technique, TUNEL staining and cell viability of prostate cells in rats was detected in different cells under radiation dose. Results of small doses of radiation although low apoptosis rate, but higher (P lt;0.01), high-dose Radiation apoptosis rate was significantly higher than low-dose radiation group and the control group (P lt;0.01), and visible hypodiploid peak. and the control group, low dose radiation group cell activity decreased (P lt;0.05), high dose radiation group cell viability decreased significantly (P lt;0.01). Conclusion Low dose radiation on rat prostate cell injury is not lethal, caused only a small amount of apoptosis, a large number of high-dose radiation can cause cell apoptosis. [Keywords:] radiation; prostate; cell apoptosis Ionizing radiation of radionuclides in the outside world as a toxin gene, can induce apoptosis in a variety of biological cells, the application of ionizing radiation-induced apoptosis in immune cells and blood vessels have endothelial cells has made important progress in other fields, but the radiation on rats apoptosis of prostate cells has not been reported (1,2). In this study, using flow cytometry (FCM), TUNEL staining and cell viability test, revealing different doses of -ray radiation on the primary culture of rat prostate cells effects. 1 Materials and methods 1.1 Experimental animals and age group 10 ~ 20 w 30 male Wistar rats by the Changchun High-tech Medical Experimental Animal Center. Animals were divided into high-dose radiation group (with colloidal 32P