4 kinds of Schisandra lignan compounds by high performance liquid chromatography.docVIP

 PAGE \* MERGEFORMAT 20 4 kinds of Schisandra lignan compounds by high performance liquid chromatography Study: You can become friends, while Qiang, Kang Hongling, in bright, Wu Xiaofang, Pei-Shi [Abstract] Objective To establish an HPLC method for simultaneous determination of 4 Schisandra lignan compounds, methods of analysis. Composition and method of inspection ratio of mobile phase, detection wavelength, column temperature, flow rate and sample volume on the determination influence the optimum chromatographic conditions: mobile phase of acetonitrile - water gradient program was: 0 ~ 25 min of acetonitrile: water = 50:50 (V / V); 25 ~ 35 min linear change to 70:30 (V / V), and keep the 5 min; 40 ~ 44 min and then linearly to 80:20 (V / V); DAD detector at a wavelength of schisandrin, Deoxyschizandrin and Schisandrin B 216 nm, Schisandra ester A 222 nm; column temperature 25 ℃; flow rate of 0.8 ml / min; injection volume 10.0 μl. results in the best chromatographic conditions, the standard curve of 4 compounds, good linear relationship, the correlation coefficient was 0.999 4 0.999 8, each component of the detection limit of 0.04 ~ 0.05 μg / ml, average recovery was 90.23 ~ 95.24%, relative standard deviation was 3.2% ~ 4.9%. Conclusion The method is sensitive, accurate, and realized 4 kinds of lignans compounds determined simultaneously. [Key Words] high performance liquid chromatography; Schisandra; lignans Schisandra Schisandra is Magnoliaceae ripe fruit, with the convergence Guse, Qi Sheng Jin, kidney and heart effect Ning. Schisandra is mainly produced in Jilin, Liaoning, Heilongjiang, has high medicinal value [1] . to establish an efficient, rapid and accurate determination of effective components in Schisandra method of detecting and tracking the quality of Schisandra [2,3], the significance will be very important, but also for standardized Schisandra medicinal plant-based quality evaluation available basis. now at home and abroad for the