8BrcAMP combined with cisplatin in A549 lung adenocarcinoma cells A preliminary study.docVIP

 PAGE \* MERGEFORMAT 19 8BrcAMP combined with cisplatin in A549 lung adenocarcinoma cells A preliminary study [Abstract] Objective: To observe the 8-bromo cAMP (8 Br cAMP) and cisplatin (DDP) on human lung adenocarcinoma A549 cell growth and epidermal growth factor receptor (EGFR) expression. Methods: MTT method different concentrations of 8 Br cAMP, DDP, and the two drugs for the treatment of human lung adenocarcinoma A549 cells the difference of cell proliferation; application RT PCR detection of EGFR mRNA expression level; Western blot detection of EGFR protein expression level; use flow cytometry cell cycle changes. Results: 8 Br cAMP and DDP could inhibit the proliferation of A549 lung adenocarcinoma cells, and a concentration and time dependent; DDP combined 8 Br cAMP inhibition concentration was significantly higher than the corresponding monotherapy group (P lt;0.05), showing a sum of two anti-tumor effect of .8 Br cAMP can be reduced in human lung adenocarcinoma A549 cells the expression of EGFR mRNA and protein .8 Br cAMP to cell cycle arrest in G2 / M phase (P lt;0.05). Conclusion: 8 Br cAMP, DDP could inhibit A549 cell proliferation .8 Br cAMP can significantly increase the cytotoxicity of cisplatin to enhance effect of chemotherapy, and cells were arrested in G2 / M phase .8 Br EGFR expression can be reduced cAMP can be reversed to some extent of the cancer cells. [Keywords:] 8 Br cAMP; lung cancer cell A549; epidermal growth factor receptor; protein kinase A [Abstract] Objective: To study the effect of 8 Br cAMP with cisplatin (DDP) on human pulmonary adenocarcinoma cell A549 growth and the expression level of EGFR in vitro. Methods: Proliferation ability and cell cycle changes of A549 cells were detected by MTT method and flow cytometry. The mRNA levels of EGFR were determined by RT PCR. The protein expression of EGFR were valued by Western blot. Results: Both DDP and 8 Br cAMP could inhibit the proliferation of A549 cells in vitro by a