About Tongbiling on rheumatoid arthritis synovial cells of vascular endothelial growth factor mRNA of.docVIP

 PAGE \* MERGEFORMAT 12 About Tongbiling on rheumatoid arthritis synovial cells of vascular endothelial growth factor mRNA of Of: Huang Qingchun, storage Massive Relay Gang, Shen Ying, Chen Jifan, Chen Sing, Wang Jie [Abstract] Objective To observe Tongbiling on cultured rheumatoid arthritis (RA) synovial cell vascular endothelial growth factor (VEGF) mRNA expression, and to explore its possible mechanism. Methods line of RA patients taking joint endoscopic surgery digestion and separation of knee joint synovial tissue cultured synovial cells are grouped. Blank control group: saline +10% FBS containing DMEM culture Tongbiling low dose: 50 mg / L +10% FBS Tongbiling DMEM culture Tongbiling high dose group: 200 mg / L Tongbiling DMEM +10% FBS culture. isolation of total RNA, reverse transcription - polymerase chain reaction (RT-PCR) method to detect the expression of VEGF mRNA . Results Tongbiling high and low dose group compared with the expression of VEGF mRNA was significantly decreased (P lt;0.05). Conclusion Tongbiling RA synovial cells can be reduced VEGF mRNA expression levels of VEGF by inhibiting the expression of which can reduce the synovial shade proliferation. [Keywords:] Tongbiling, rheumatoid arthritis, synovial cells, vascular endothelial growth factor Abstract: Objective To study effect of Tongbiling (TBL) on VEGF mRNA expression in RA synovlcytes cultured in vitro and explore its mechanism. Method Synovial cells from the knee joint in patient with RA were digested, divided and separately cultured after arthroscopy. Blank controls: DMEM culture containing 10% FBS in saline, Low-dose admission group of TBL: DMEM culture containing 10% FBS in 50 mg / L TBL, High-dose admission of TBL: DMEM culture containing 10% FBS in 200 mg / L TBL. RNA were distilled. Expression of VEGF mRNA were detected with RT-PCR. Result Compared with control group, expression of VEGF mRNA in low and high-dose admission groups of TBL both decreased (P lt;0.05). C