About two ways to compare the efficiency of extraction of glycyrrhizic acid.docVIP

 PAGE \* MERGEFORMAT 9 About two ways to compare the efficiency of extraction of glycyrrhizic acid [Abstract] Objective To compare the water extraction of acid and ammonia content of glycyrrhizic acid changes. Methods orthogonal 4 factors and 3 levels to choose the best extraction conditions (including the amount of water, extraction times, boiling time, grinding time, solvent volume, shaking time, ammonia concentration), by ultraviolet spectrophotometry at a wavelength of 252 nm in the determination of glycyrrhizic acid content. Results of water per 100 g glycyrrhizic acid meal optimal conditions for extraction: each volume of water 2 000 mL, extracted 2 times, boiled 5 min, crushed 20 s, optimal conditions for the ammonia method: each plus 0.4% ammonia 2 000 mL, oscillator 2 h, extracted 2 times. final extraction rate of 32% ammonia, water, acid extraction rate of 26%. Conclusions Under optimal conditions, extraction of ammonia and extraction of glycyrrhizic acid was significantly higher than that of pure water acid. [Keywords:] licorice, glycyrrhizic acid, water, acid, ammonia, orthogonal test Keywords:: Radix glycyrrhiza, Glycyrrhizic acid, decoct method, Ammonia-water method, orthogonal design For the legume licorice licorice (Glycyrrhiza uralensis Fisch), Glycyrrhiza (Glycyrrhizainflata Bat.) Or Glycyrrhiza glabra (Glycyrrhizaglabra L.) of the dried roots and stems. Licorice contains glycyrrhizin in the main, glycyrrhetinic acid, flavonoids, alkaloids, amino acids and other chemical components, with a wide range of physiological activities. Glycyrrhizic acid in licorice is the most important chemical composition (the content of the highest, up to 14%), with detoxification , anti-inflammatory, anti-allergy treatment of ulcers, antitussive, anti-tumor and prevention of viral hepatitis, hyperlipidemia and the role of diseases such as cancer [1]. In this experiment the orthogonal design to compare 2 different glycyrrhizic acid extraction metho