Accompanied by 6; 9 chromosomal translocation in acute myeloid leukemia in patients with DEK-CAN fusion gene expression analysis.docVIP

 PAGE \* MERGEFORMAT 9 Accompanied by 6; 9 chromosomal translocation in acute myeloid leukemia in patients with DEK-CAN fusion gene expression analysis Author: Allen Wang, Tong Wang, Feng Xu, Gang Yan, Wang Jie Abstract This study aimed to explore the acute myeloid leukemia (AML) in patients 6; 9 chromosomal translocation and DEK-CAN fusion gene expression and clinical significance of the relationship between. Patients with short-term cultured bone marrow cells prepared according to conventional methods of chromosomes, using R-banding karyotype analysis techniques. Reverse transcription-nested polymerase chain reaction (RT-nest-PCR) on four cases of AML in patients with bone marrow or peripheral blood mononuclear cells, DEK-CAN fusion gene expression were analyzed, and three of them routine allogeneic bone marrow transplantation (allo -BMT) in patients with dynamic follow-up. The results showed that: 4 cases of acute myeloid leukemia patient, t (6; 9) (p23; q34) translocation; four cases of newly diagnosed, and then hair and a complete remission in patients with different degrees were detected in DEK-CAN fusion gene, accounting for 100% ; of these newly diagnosed, and then the peak of expression was significantly enhanced, complete remission decline; 3 patients after allo-BMT patients 1-24 months, all expressed DEK-CAN mRNA. Clinical data show four cases of AML patients, 2 patients in CR relapsed after 1-24 months (of which 1 underwent allogeneic bone marrow transplantation), death, and the remaining two cases of complete remission in the treatment of successively received allogeneic bone marrow transplant, is now alive. Conclusion: DEK-CAN gene is the molecular basis of pathogenesis of AML to detect DEK-CAN fusion gene for AML diagnosis, efficacy and prognosis of great significance. Keywords: acute myeloid leukemia Analysis of DEK-CAN Fusion Gene Expression in Acute Myeloid Leukemia Patients with 6; 9 Chromosome Translocation Ab