Acellular bovine cancellous bone combined bone marrow stromal stem cells to repair bone defect.docVIP

 PAGE \* MERGEFORMAT 7 Acellular bovine cancellous bone combined bone marrow stromal stem cells to repair bone defect Author: Wang Chao Sun Jianhua, SHI Chen-hui Dong Jinbo Liu Zhengyu Qin Gang [Abstract] Objective: To develop the ideal bone to repair bone defect material for bone defect repair in the field of experimental basis for clinical applications. Methods: In vitro expansion of rabbit bone marrow stromal stem cells it with acellular bovine cancellous bone composite culture. Caused by the middle 10 mm of rabbit radial bone defect model, sub-composite cell group, simple materials group, blank control group. By gross observation, radiographic examination, histological observation of the repair of bone defects. Results: The acellular bovine cancellous bone combined bone marrow stromal stem cells to repair bone defect, 12 weeks, the defect was completely replaced by new bone, bone marrow cavity completely smooth, better than the pure materials, the restoration group effect, simple materials group was better than the control group . Conclusion: The acellular bovine cancellous bone into the bone has a good ability, it is possible ideal bone scaffold material. [Keywords:] bone repair materials; acellular bovine cancellous bone; Tissue Engineering 〔 Abstract〕 Objective To prepare an ideal scaffold material for the repair of bone defect, for the clinical use in the bone reconstruction field. Methods Rabbit marrow stem cells (MSCs) were cultured and expanded in vitro. The MSCs were mixed with acellular bovine cancellous bone (ABCB). The rabbits were prepared as model of bone defects, divided into 3 groups: named complex group, acellular bovine cancellous bone group, and blank control. The animals were sacrificed and new bone regeneration in the defects was investigated by gross specimen inspection, X-ray examination and histological observation. Results At 12 weeks, the radial defects repaired by named complex group, and their marrow cavities formed, which is