Actinomyces SPRI70014 herbicide constituents of.docVIP

 PAGE \* MERGEFORMAT 10 Actinomyces SPRI70014 herbicide constituents of Author: Guxue Bin Xu Ping Ye Gao Yi Zhang Lei Ni Weiwei Li-Ming Tao [Abstract] one from Jiangxi soil, code-named SPRI  70014 Actinomycetes fermentation product has a strong herbicide activity, this paper, solvent extraction, silica gel column chromatography and the preparation by means of HPLC in the fermentation product of the activity of group active track points were separated more than 98% purity was obtained pure compounds, through the physical and chemical properties and spectral analysis, their structures were identified as 2  [2  (3,5-dimethyl-oxygen   2  cyclohexyl)  6  O   4  base tetrahydropyran]  acetamide. And use herbicide bioassay standardized operation method (SOP) a preliminary evaluation of the herbicidal activity of the compound. [Keywords:] Actinomycetes; secondary metabolites; acetamide; herbicide activity ABSTRACT To explore the herbicidal active component in fermentation of a soil actinomycete SPRI  70014, a compound was isolated and purified by using solvent extraction, silica gel column chromatography and preparative HPLC. The separation procedure was traced by herbicidal activity. By means of physico  chemical properties and spectral analyses, the structure of the compound was determined as: 2  [2  (3,5  Dimethyl  2  oxo  cyclohexyl)  6  oxo  tetrahydro  pyran  4yl]  acetamide. The herbicidal activity of the compound was assayed by SOP method. KEY WORDS Actinomycete; Secondary metabolites; Acetamide; Herbicidal activity The use of micro-organisms and bio-active substances produced by the source as a new pesticide research and development of researchers around the world is being a great attention [1]. This is because the natural decomposition of natural material and not generally susceptible to pollution of the environment, but also can make use of microbial growth and reproduction of almost the same as the e