Acupuncture on rats with adjuvant arthritis synovial mast cell degranulation and tryptase expression.docVIP

 PAGE \* MERGEFORMAT 29 Acupuncture on rats with adjuvant arthritis synovial mast cell degranulation and tryptase expression OF: He Tianfeng, detached, Lilian Bo, Yang Wenjia, Zhu Jun, Yun-Fei Chen [Abstract] Objective: To observe the adjuvant acupuncture on pathological changes in synovial tissue, synovial mast cell degranulation and tryptase expression to explore the function of mast cells in rats with adjuvant arthritis treated with acupuncture inflammation. Methods: 46 male Wistar rats were randomly divided into normal control group (n = 16, model group (n = 15 and the acupuncture group (n = 15. In addition to the normal control group, the other rats by footpad Department of Freund complete adjuvant injected 0.1 mL of adjuvant arthritis model established. acupuncture acupuncture both Zusanli day, hanging bell, Shenshu, every 15 min, a total of 8 times. treatment, normal control group and model group than non-acupuncture, the other treatments with acupuncture. based modeling on the day of experiment on day 0 and then every 3 days and body weight of rat paw volume was measured and recorded. the end of treatment After taking all the rat right ankle after the synovial tissue, HE staining of synovial pathology scale of 5, based on evaluation of synovial tissue pathological changes in law, toluidine blue staining and the number of synovial mast cell degranulation case, the immune histochemical detection of tryptase in the synovial tissue. Results: Compared with model group, acupuncture group were significantly increased body weight after treatment (P lt;0.05, significantly reduced paw volume (P lt;0.01. HE staining showed that acupuncture can significantly reduce the synovial tissue of adjuvant arthritis in rats with inflammatory cell infiltration, synovial hyperplasia and synovial fibrosis and other pathological manifestations (P lt;0.05, toluidine blue staining, and the normal control group, model group, the number of synovial mast cells and degranulation