Activation of Chk1 induced G2 - M phase arrest in the regulation of K562-A02 cells in the mechanism of drug resistance.docVIP

 PAGE \* MERGEFORMAT 10 Activation of Chk1 induced G2 / M phase arrest in the regulation of K562/A02 cells in the mechanism of drug resistance Author: Wang Haiyan Zhang Min ZOU Guo-Ming Tang Xiaoqiong swimming Zhao Gang WU Yao-hui Abstract This study investigated the activation of Chk1 on leukemic cell cycle and apoptosis to explore the Chk1 regulation of tumor cell resistance mechanisms. With chronic myeloid leukemia cell line K562 and its resistant cell line K562/A02 (adriamycin resistant) as the research object, and adriamycin were incubated with a cell cycle distribution by flow cytometry, RT-PCR detection of Chk1mRNA expression level, Western blot detected after transfection of Chk1 phosphorylation levels; targeted Chk1shRNA inhibited the expression of Chk1 after adriamycin with the role of flow cytometry after cell apoptosis. The results showed that: Adriamycin-induced K562/A02 cells were arrested in G2 / M phase cell percentage (54.12 ± 0.57)%, significantly higher than K562 cells (36.99 ± 1.28)%; Chk1mRNA expression level in K562 cells and K562/A02 was no significant difference between the; Chk1 phosphorylation levels in K562/A02 cells 0.79 ± 0.56, in the K562 cells 0.27 ± 1.47, the differences were statistically significant. Transfection Chk1shRNA, the two cells, Chk1 phosphorylation levels dropped significantly. Transfected group K562, K562/A02 cell apoptosis rates were empty vector transfected group 1.30 times and 3.84 times. Conclusion: Chk1 regulates the activation level of K562/A02 cells to doxorubicin sensitivity. Keywords: Chk1 G2 / M phase arrest K562/A02 cells, K562 cells, RNA interference-resistant leukemia cells Mechanism of G2 / M Blockage Triggered by Activated-Chk1 in Regulation of Drug-resistance in K562/A02 Cell Line Keywords:Chk1; G2 / M arrest; K562 cell; K562/A02 cell line; RNA interference; leukemia cell drug-resistance Cell cycle G2 / M checkpoint is to monitor the cell into the mitotic phase of