Acute myeloid leukemia cell culture supernatant of CD4 and CD8 T cell proliferation and apoptosis.docVIP

 PAGE \* MERGEFORMAT 5 Acute myeloid leukemia cell culture supernatant of CD4 and CD8 T cell proliferation and apoptosis Author: Wang Xingbing, Liu Juan, He Yanli, Gu Jun Xia, Zheng Jine, Yao Junxia, Yang Jing, Li Xiaoqing, Huang Shi-Ang [Abstract] In order to study different acute myeloid leukemia (AML) cell line culture supernatant of CD4 and CD8 T-cell proliferation and apoptosis, and to explore the formation of AML immunosuppressive mechanism, three kinds of AML cell lines (HL -60, NB4, and U937) and the general culture supernatant fluid by mixing different proportions for the training CFSE staining lymphocytes. Anti-CD3 antibody and anti-CD28 antibodies to stimulate the three days, marking 7AAD and the corresponding fluorescent antibody. The use of flow cytometry in different T cell subsets in CFSE fluorescence intensity and 7AAD expression rate of change, analyze the changes in proliferation and apoptosis. The results showed that: three kinds of AML there are two kinds of cell lines (HL-60 and NB4) supernatant significantly inhibited CD4 T cells and CD8 T cell proliferation, and increased with increasing concentration. Similarly, HL-60 and NB4 cell line culture supernatant after stimulation can also inhibit apoptosis of CD4 T cells, but CD8 T cells after stimulation with no significant effect on apoptosis. On the contrary, HL-60 and NB4 cell line supernatant significantly increased the proliferation of CD8 T cell apoptosis. Conclusion: AML cell line supernatant of the CD4 T cells and CD8 T cell proliferation and the inhibition of the proliferation of CD8 T cells induced apoptosis might be defective immune function in patients with AML one of the mechanisms. [Keywords:] Acute myeloid leukemia Effects of Acute Myeloid Leukemia Cell Supernatant on the Proli-feration and Apoptosis of CD4 and CD8 T Cell Subsets Abstract To study the effects of supernatant derived from acute myeloid leukemia (AML) cell lines on proliferation and apoptosis of CD4 and CD8