Acute promyelocytic leukemia patients with minimal residual disease detection and clinical significance of.docVIP

 PAGE \* MERGEFORMAT 12 Acute promyelocytic leukemia patients with minimal residual disease detection and clinical significance of Author: Ren-Quan, Tang Hua, Bi can be red, Zhang Yukun, Zhao Liangyu, GUO Gui-month, Liu Xiu-lan, JIANG Feng-Qin, Liu Chuan-Fang, PENG Jun, TIAN Zhi-Gang [Abstract] Objective To determine the complete remission of acute promyelocytic leukemia patients with minimal residual disease (MRD), while the guidance of its prognostic value. The establishment of reverse transcriptase PCR (RT / PCR) for detection of APL patients with PML / RARα. Results RT-PCR, a sensitive and reproducible. APL patients before RT-PCR positive rate was 92.8% (39/42), while retinoic acid or chemotherapy-induced complete remission after the MRD-positive rate was 56.7% (21/37). In addition, MRD-positive patients with post-remission relapse APL and APL can be used as post-remission predict relapse indicators. At the same time to help guide clinical treatment to prevent recurrence of the consolidation. Conclusions RT-PCR to detect MRD in patients with APL, and as a predictor of relapse indicators. [Keywords:] acute promyelocytic leukemia; minimal residual disease; RT / PCR Leukemia complete remission (CR) after the minimal residual disease (MRD) has been confirmed with the relapse [1]. MRD detection has become a guide for further post-remission chemotherapy, an important indicator of [2]. In the practice of blood diseases, MRD detection after bone marrow transplantation is the best example of RT-PCR detection of chronic myeloid leukemia (CML), BCR-ABL fusion gene [3]. Acute promyelocytic leukemia (APL) with specific chromosomal t (15; 17) (q22; q21) translocation is characterized by [4]. Recently, many laboratories found that t (15; 17) translocation, the adoption of the PML gene on chromosome 15 and the RARα gene on chromosome 17 resulting fusion genes side by side PML / RARα to form [5]. According to PML breakpoints