Adenovirus-mediated brain-derived neurotrophic factor gene in cultured Schwann cells.docVIP

 PAGE \* MERGEFORMAT 22 Adenovirus-mediated brain-derived neurotrophic factor gene in cultured Schwann cells Abstract observations of adenovirus-mediated human brain-derived neurotrophic factor (brain-derived neurotrophic factor, BDNF) gene in cultured Schwann cells (Schwann cell, SC) in the expression of the optic nerve injury and regeneration of tissue engineering to provide reliable used to seed cells. Methods: In 293 cells cultured amplified BDNF adenovirus (adenovirally delivered BDNF, Ad-BDNF), measured with plaque formation assay titer of infection. SD rats were cultured in vitro-derived SC, with Ad-BDNF infection in cultured SC, with the reverse transcription - polymerase chain reaction (reverse transcriptase polymerse chain reaction, RT-PCR) detection of BDNF mRNA expression by Western blot (immunoblot assay, Western Blotting) and enzyme-linked immune response test (enzyma-linked immumosorbent assay, ELISA) in culture supernatant BDNF expression. Results: Ad-BDNF by the amplified obtain a higher titer of recombinant adenovirus vector infection. Under normal culture conditions without the Ad-BDNF infected SC low expression BDNF, after the SC infected 3d its expression was significantly increased BDNF expression level increased with time, and be able to 21d after infection to maintain high expression of the trend. Conclusion: Ad-BDNF to BDNF-mediated gene transfer to cultured SC, the latter a longer period can be highly expressed BDNF. 【Key Words】 optic nerve injury 0 Introduction The optic nerve as a special division of the central nervous system, its local micro-environment after injury neurotrophic factor (neurotrophic factor, NTF), in its repair and regeneration play an important role in the process of brain-derived neurotrophic factor (brain-derived neurotrophic factor , BDNF) is one of the earlier affirmed [1]. In vivo and in vitro experiments have confirmed, BDNF can be maintained to promote peripheral neural crest a