Advanced glycation end products on human monocyte expression of EMMPRIN.docVIP

 PAGE \* MERGEFORMAT 11 Advanced glycation end products on human monocyte expression of EMMPRIN [Abstract] Objective: To explore the advanced glycation end products (AGEs) on human monocyte extracellular matrix metalloproteinase inducer (EMMPRIN) expression. Methods: In cultured human monocyte cell line, respectively, with different concentrations of AGEs interfere with 24 h and at the same time, the concentration of AGEs interfere with different points to 1640 and the corresponding concentration of bovine serum albumin as the control group. Reverse transcription-polymerase chain reaction detection of EMMPRIN mRNA expression levels. Results: AGEs interfere with human monocyte cell EMMPRIN mRNA levels compared with control group the difference was statistically significant (P amp;lt;0.05); and its expression in a time-and concentration-dependent decrease (P amp;lt;0.05). Conclusion: AGEs with time and concentration-dependent manner to reduce human monocyte EMMPRIN mRNA expression. [Keywords:] advanced glycation end products; human monocyte cell; extracellular matrix metalloproteinase inducer; reverse transcription polymerase chain reaction; Diabetes [Abstract] Objective: To investigate the effects of advanced glycosylation end products (AGEs) on the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) in cultured human monocytes. Methods: The cultured human monocytes were incubated with AGEs (50,100,200, 400 mg * L-1) for 24 h or 200 mg * L-1 AGEs for 8,16,24 and 48 h respectively, which were incubated with 1640 medium and BSA as a control. The mRNA expression of EMMPRIN in human monocytes was analyzed by RT-PCR. Results: Compared with that in cells incubated with 1640 and BSA, the level of EMMPRIN mRNA in cells treated with AGEs decreased significantly in a concentration-dependent and time-dependent manner. Conclusion: The AGEs inhibit the expression of EMMPRIN in cultured human monocytes. [Keywords:] Advanced glycation end products; Hum