Advanced glycation end products on human glomerular mesangial cells of connective tissue growth factor and fibronectin gene expression in.docVIP

 PAGE \* MERGEFORMAT 5 Advanced glycation end products on human glomerular mesangial cells of connective tissue growth factor and fibronectin gene expression in [Abstract] Objective: To investigate the in vitro culture conditions, advanced glycation end products (AGEs) in human glomerular mesangial cells of connective tissue growth factor (CTGF) and fibronectin (FN) gene expression. Methods: Preparation of different concentrations of AGEs in vitro intervention in mesangial cells and the same concentration of AGEs interfere with cells at different times. Application of RT  PCR detection of cells in the mRNA levels of CTGF and FN. Results: With the increased concentration of AGEs to intervene or interfere with the time extension of mesangial cells, and FN mRNA levels of CTGF compared with control group increased significantly. Conclusion: AGEs can lead to human mesangial cells, the expression of CTGF and FN mRNA increase in the incidence of diabetic nephropathy, development play a role. [Keywords:] advanced glycation end products; glomerular mesangial cells; diabetic nephropathy; connective tissue growth factor; fibronectin Diabetic nephropathy (diabetic nephropathy, DN) is a chronic microvascular complication of diabetes, characterized by pathological changes in glomerular mesangial expansion, capillary basement membrane thickening, extracellular matrix accumulation which eventually led to a large number of glomerular hardening. A large number of studies have shown that advanced glycation end products (advanced glycation end products, AGEs) in the DN of the occurrence and development played an important role in [1], which can induce over-expression of various growth factors which accelerate the renal fibrosis. This study was prepared by AGEs in vitro studies on human glomerular mesangial cells of connective tissue growth factor (CTGF), fibronectin (FN) expression to explore AGEs in glomerular fibrosis. 1 Materials and methods 1.1 Materials bovine serum al