Aging mice and the thymus microenvironment.docVIP

 PAGE \* MERGEFORMAT 12 Aging mice and the thymus microenvironment [Abstract] Objective To observe the process of aging in the mouse thymus microenvironment of thymic epithelial cells, macrophages, and the morphological changes in the microenvironment of the cells associated with thymic aging of interleukin (IL) 6 of the changes, and then judge the thymus micro-environment changes and the relationship between age of rats. Methods HE and electron microscopy methods 1,6,12 and 16 months old mouse thymus morphology were observed by immunohistochemical methods on four different months in the mouse thymus microenvironment IL 6 expression were analyzed. Results With aging in mice, thymus microenvironment mainly as different degrees of thymic epithelial cells and macrophages in the destruction of the increase. thymic microenvironment in mice of different months in the secretion of IL 6 varying intensity. Conclusion Rat thymus micro-environment old growth with changes evident. thymic microenvironment in the strength of IL 6 expression increased in small Shushu age was positively correlated. [Keywords:] thymus, aging, IL 6, mice Thymic microenvironment, stromal cells from the thymus (thymic stroma cell, TSC, including epithelial cells, macrophages, fibroblasts, etc., extracellular matrix (ECM and their secreted soluble molecules, micro-environment for differentiation and thymic T cells development provides the most suitable living environment (1). murine age-related degenerative changes bound to affect the function of the thymus microenvironment. This study was designed to observe by means of some experiments in mice during aging thymus microenvironment changes and age-related thymus cells with interleukin (IL) 6 expression. 1 Materials and methods 1.1 Animals 1,6,12 and 16-month-old BALB / c mice were each 10 by the Second Clinical Medical College of Harbin Medical University Experimental Animal Center. 1.2 Methods 1.2.1 Reagents and apparatus