Alcohol-induced liver fibrosis in the study of the expression of Smad4.docVIP

 PAGE \* MERGEFORMAT 5 Alcohol-induced liver fibrosis in the study of the expression of Smad4 Author: Wu Ling-Kang Xiao-Qi Wang Zili Ye Wei Ren Abstract: Objective To explore the Smad4 in alcoholic liver disease in liver tissue expression and distribution of alcoholic liver disease revealed some of the pathogenesis. Methods Masson staining of liver fibrosis in alcoholic liver fibrosis study, using immunohistochemical study Smad4 molecules in alcoholic liver disease liver tissue, using RT-PCR were conducted to investigate the expression of Smad4 in the nucleic acid level and distribution. Results In normal liver tissue and liver alcohol early, Smad4 expression is negative, alcoholic liver fibrosis stage of Smad4 expression was positive, mainly in the periportal areas, wall-week gap, fiber spacing and other non-parenchymal cells, and further PCR method to study and found that the normal group and alcoholic liver compared with the late group, which Smad4 expression levels were significantly different. Conclusion Smad4 involved in alcohol-induced liver fibrosis. [Keywords:] alcoholic liver disease; liver fibrosis; Smad4; TGF-beta [Abstract] Objective To investigate the expression and role of Smad4 in ALD. Methods imumnohistochemistry and reverse transcription polymerase chain reaction was used. Results There is no expression of Smad4 in normal liver and liver of earlier period ALD. Smad4 significantly increase in liver of late period ALD. They mainly distributed in portal area, perisinusoidal space and fibrous septa. Conclusion: Smad4 might be a important factor of fibrogenesis in late period of Alcoholic Liver Disease. [Keywords:] Alcoholic Liver Diseas Smad4 liver fabrosis TGF-beta In recent years, along with alcohol consumption increases the incidence of alcoholic liver disease showed an upward trend, alcohol-induced liver disease, including alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis cirrhosis, the pathogenesis of the disease is n