Alzheimers disease model rat hippocampus Bcl2 Bax expression and the intervention of seven fu drink.docVIP

 PAGE \* MERGEFORMAT 15 Alzheimer’s disease model rat hippocampus Bcl2 Bax expression and the intervention of seven fu drink Study: Problem and Reform Linchun Rong Xing Guihua Hu Nan Niu Ying Cai [Abstract] Objective To investigate the amyloid protein (A1 42) induced senile dementia (AD model rats Bcl 2, Bax expression and seven of their welfare intervention of drinking. Methods hippocampal A1 42 stereotactic injection preparation of condensed matter Experimental animal model of AD, drug intervention, after 4 w real time quantitative PCR, Bcl 2 mRNA and Bax mRNA expression. by immunohistochemical staining and computer image analysis technique in determining the hippocampus Bcl 2, Bax protein positive goal of integrated optical density . The results with the sham operation group, model group, Bax mRNA and protein expression level was significantly increased, while Bcl 2 mRNA and protein expression was significantly reduced. Compared with model group, hydrochloric donepezil group, seven fu drink each dose group Bax mRNA and protein was significantly reduced, and seven drinks each dose group blessing Bcl 2 mRNA and protein expression was significantly increased (P lt;0.05. Conclusion hippocampus after injection of A1 42 expression in hippocampus was significantly increased Bax, Bcl 2 expression was significantly reduced, seven Fu Bax expression in the hippocampus inhibit drinking, and promote the expression of Bcl 2, anti-drink for the seven AD Fu one of the mechanisms. [Keywords:] Alzheimer’s disease, seven fu drink, immunohistochemistry, apoptosis, real-time quantitative PCR Studies have shown that neuronal apoptosis is an important mechanism of AD occur (1). Recent studies have confirmed that some of the traditional Chinese medicine may be AD hippocampus by inhibiting Bax gene expression and the promotion of Bcl 2 gene expression, reduced neuronal apoptosis, to the role of anti-AD (2 ~ 4). VII classical name for the traditional blessing drinking pa