All-trans retinoic acid and oxaliplatin in human gastric cancer BGC823 induced apoptosis in synergy.docVIP

 PAGE \* MERGEFORMAT 13 All-trans retinoic acid and oxaliplatin in human gastric cancer BGC823 induced apoptosis in synergy Of: Shao should move, Zheng Yuling, Fan Qingxia, Zhao Peirong [Abstract] Objective To observe the all-trans retinoic acid (ATRA) and oxaliplatin (L OHP) in human gastric cancer BGC 823 cells. Methods ATRA and L OHP were used to separate and combined effects on human gastric cancer BGC 823 cells, MTT assay using cell proliferation was observed under inverted microscope changes in cell morphology; apoptosis rate by flow cytometry and cell cycle; immunocytochemical detection of BGC 823 cells, Bcl 2 and Survivin protein expression. Results ATRA After the role of the proliferation of BGC 823 cells was inhibited, the cells changed, apoptosis was significantly increased; and L OHP combined with later, the effect of strengthening. gastric cancer BGC 823 cells, Bcl 2 and Survivin protein expression in both ATRA after effects inhibited, ATRA and L OHP after the combined effect of the positive rate of expression is further reduced. Conclusion ATRA can inhibit the growth of gastric cancer cells BGC 823, and L OHP combined with a synergistic effect, which may be associated with downregulation of Bcl 2 and Survivin protein expression. [Keywords:] all-trans retinoic acid; oxaliplatin; gastric cancer BGC 823 cells; apoptosis; Bcl 2; Survivin ABSTRACT: Objective To observe the effects of all trans retinoic acid (ATRA) and oxaliplatin (L OHP) on the proliferation of human gastric cancer BGC 823 cells. Methods Human gastric cancer cells BGC 823 were treated with ATRA and / or L OHP, respectively . The cell proliferative activity was assessed by MTT assay. Cell morphological changes were observed under inverted microscope while apoptosis rate and cell cycle were assayed by flow cytometry. The expressions of Bcl 2 and Survivin protein were detected by immunocytochemistry. Results The proliferation of the cells treated with ATRA was inhibited obviou