All-trans retinoic acid increases ectopic endometrial stromal cells GJIC function of the mechanism.docVIP

 PAGE \* MERGEFORMAT 9 All-trans retinoic acid increases ectopic endometrial stromal cells GJIC function of the mechanism Author: Liu energy, YAO Zhen-wei, TANG Liang-dan, Bian Duhong [Abstract] Objective: To explore the all-trans retinoic acid (ATRA) on human ectopic endometrial stromal cells, gap junctional intercellular communication (GJIC) function of regulation and its mechanism. Methods: The human endometriosis (EMs) in patients with interstitial cells, using 0.1,1,10 μ mol / L ATRA in 24,48,72,96 and 120 h respectively, and taking set the blank control group. using laser confocal microscopy ectopic endometrium of the GJIC function of stromal cells, and detection and GJIC function closely related to Cx43, Cx26 and Cx32 gene and protein expression. Results: After ATRA treatment, 1 μ mol / L and 10 μ mol / L treatment group between the ectopic endometrium stromal cells markedly enhanced fluorescence recovery; 0.1 μ mol / L treatment group of ectopic endometrial stromal cells, no significant change in fluorescence recovery. without ATRA treatment of ectopic endometrial stromal cells in the non-Cx43, Cx26 and Cx32 in mRNA and protein expression; by 1,10 μ mol / L ATRA treatment, ectopic endometrial stromal cells was detected in Cx43 mRNA and protein expression, but no Cx26 and Cx32 expression of the mRNA and protein. stromal cells of the GJIC function and dephosphorylated Cx43 protein expression. Conclusion: ATRA can selectively induce dephosphorylated Cx43 gene and its protein expression, and thus increase ectopic endometrial stromal cells GJIC function; ATRA acid may be a potential drug to treat EMs . [Keywords:] all-trans-retinoic acid; mesenchymal cells; gap junction intercellular communication; endometriosis [Abstract] AIM: To explore the possible mechanism underlying the up  regulation on gap junctional intercellular communication (GJIC) by all  transretinoic acid (ATRA) in human endometriotic stromal cells. METHODS: Ectopic endometrial stroma