Amicoumacin B of the MG63 cells bmp2 mRNA and protein expression in vitro.docVIP

 PAGE \* MERGEFORMAT 11 Amicoumacin B of the MG63 cells bmp2 mRNA and protein expression in vitro Author: Yang Zhaoyong Zhang Zhifei He Xiaobo Yang Jun Sun Wei Ding Shu-yi Zhang Yueqin Division [Abstract] Objective To study the amicoumacin B (04  5195A) of bone morphogenetic proteins factor II (BMP  2) role. Methods RT  PCR technique to study amicoumacin B cells in human MG63 osteoma of the BMP  2 transcription; application of flow cytometry analysis technology amicoumacin B on the MG63 cells of the BMP  2 expression. Results Amicoumacin B at the mRNA and protein levels can be enhanced BMP  2 transcription and expression. Concluded the first demonstration of amicoumacin B can raise the role of BMP  2. [Keywords:] Nocardia; Amicoumacin B; BMP  2; transcription; expression ABSTRACT Objective To investigate the effect of amicoumacin B on bone morphogenetic protein  2 (BMP  2). Methods The effect of amicoumacin B to bmp  2 transcription and expression in MG63 cells were studied by means of RT  PCR analysis and Flowcytometry respectively. Result Amicoumacin B can enhance the transcription and expression of BMP  2 at the level of mRNA and protein. Conclusion The activity of amicoumacin B on BMP  2 is proved for the first time. KEY WORDS Amicoumacin B; BMP  2; Transcription; Expression Osteoporosis is due to osteoclasts (osteoclast) and adult bone cells (osteoblast) imbalance between the function of co-ordination, so that more than osteolysis due to bone formation. Bone morphogenetic protein factor II (bone morphogenetic protein  2, BMP  2) in the osteoblast differentiation process play a very crucial role, in addition to bone regeneration and repair process to promote osteoblast differentiation and inhibit their apoptosis, [1,2], but also to raise and induce bone marrow stem cells to differentiate into osteoblasts and chondrocytes, through the formation of new bone calcium deposition; In addition, BMP  2 can promote osteoblas