An extraction process on the orthogonal Granule Optimization.docVIP

 PAGE \* MERGEFORMAT 8 An extraction process on the orthogonal Granule Optimization On: An Yan, Lu rabbit forest, Maxin Fei, Yin Zhou release [Abstract] Objective on safety of Granule extraction process. Methods orthogonal design to chlorogenic acid and cinnamic acid as an index, select the L9 (34) orthogonal to study alcohol concentration, alcohol consumption, extraction times, extraction time and other parameters of chlorogenic acid and cinnamic acid content. Results Pieces of feeding, increased by 9 times the amount of 70% ethanol, extracting 2 times, each 1.0 h for the optimum. CONCLUSION The process is stable and reliable, can be used for production. Keywords:] An Granule, chlorogenic acid; cinnamic acid, orthogonal design, high performance liquid chromatography Granule security by honeysuckle, Scrophulariaceae, Codonopsis, heterophylla, etc. made by the extraction processing of traditional Chinese medicine, with blood circulation, nourishing yin meridians of the efficacy of clinically used in thromboangiitis obliterans , venous thrombosis, arteriosclerosis obliterans, cerebral thrombosis and sequelae and so on. To ensure the safety and scientific Granule extraction process is reasonable, good for patients to take the test to chlorogenic acid, cinnamic acid as index, the orthogonal An experimental design method, alcohol extraction process Granule study, the optimum process. An instrument and reagent Agilent 1100 high performance liquid chromatography system (Agilent Technologies), CP225D microbalance (Germany Sartorius), KQ-500E type of medical ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.). Chlorogenic acid (Pharmaceutical and Biological Products, batch number 110753-200212), cinnamic acid (Pharmaceutical and Biological Products, batch number 110786-200403). Acetonitrile, methanol HPLC grade, all other reagents were of analytical grade. 2 Methods and Results 2.1 Orthogonal experimental design Weigh in proportion