Androgen Deficiency in Mice reactive oxygen species and Rb protein expression and the impact of aging on the heart.docVIP

 PAGE \* MERGEFORMAT 13 Androgen Deficiency in Mice reactive oxygen species and Rb protein expression and the impact of aging on the heart Study: Race beads Ruanyun Jun Zhang Li Hong-Lei Lai Wu Wen Yan [Abstract] Objective To investigate the androgen levels in mice lacking the heart of senescence. Methods The experiment is divided into normal C57BL / 6J group male (n = 8), ovariectomized group (n = 8), rat testicular feminization (tfm ) group (n = 10), aging group (n = 8). measured levels of serum testosterone concentration, separation and determination of myocardial superoxide dismutase (SOD), malondialdehyde (MDA) level and the determination of phosphatase Western blotting of Rb protein expression. Results Compared with normal group, the aging group, ovariectomized group tfm serum testosterone concentrations significantly decreased (P lt;0.01); myocardial tissue SOD activity decreased (P lt;0.05, P lt;0.01), MDA levels increased (P lt;0.01); dephosphorylated Rb protein expression was increased (P lt;0.01). and aging group, ovariectomized group and the SOD activity tfm group, MDA content and dephosphorylated Rb protein expression was no statistical difference significance. Conclusions lack of androgen levels in vivo may be through increased levels of reactive oxygen species (ROS), and proteins in mice Rb myocardial aging. [Keywords:] aging; androgen; castration; of reactive oxygen species; to phosphorylation of Rb protein [Abstract] ObjectiveTo explore the effect of androgendeficiency on myocardium senescence in mice.MethodsAt 8 weeks of age, male C57BL / 6 mice were randomly separated into control, castrated and senescence groups, each group had 8 mice. Tfm mice were used as tfm group (n = 10). Besides senescence group, allgroupswere fed for 24 w. Blood was collected for measurement of testosterone concentrations. The activity of SOD and the level of MDA in the myocardium tissues weredetermined, and the expression of dephosphorylation Rb was detec